{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T07:02:38Z","timestamp":1768719758942,"version":"3.49.0"},"reference-count":63,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monitoring of planktonic salmon louse (<jats:italic>Lepeophtheirus salmonis salmonis<\/jats:italic>) abundance and parameterization of key life\u2010history traits has been hindered by labour\u2010intensive and error\u2010prone quantification using traditional light microscopy. Fluorescence illumination has been proposed as a means of improving visualization, but prior to this study adequate investigation of the relevant fluorescence profiles and measurement conditions has not been undertaken. We investigated the fluorescence profiles of <jats:italic>L.\u00a0salmonis<\/jats:italic> and non\u2010target copepod spp. with excitation and emission matrices (200\u2013600\u00a0nm) and identified unique fluorescence signals. Fluorescence microscopy using excitation wavelengths of 470\u00a0\u00b1\u00a040\u00a0nm, and emission wavelengths of 525\u00a0\u00b1\u00a050\u00a0nm, showed that after 90\u00a0days of formalin storage salmon lice have a mean fluorescence intensity that is 2.4 times greater than non\u2010target copepods (copepodid and adult stages). A 7\u2010day heat treatment of 42\u00b0C in formalin increased the difference between salmon louse copepodids and non\u2010target copepods to a factor of 3.6, eliminating the need for prolonged storage. Differences in the fluorescence signal and endogenous fluorophores were investigated with respect to variation in sea lice species, age, stage and host fish origin. Under the conditions outlined in this paper, the fluorescence signal was found to be a reliable means of visualizing and differentiating salmon lice from non\u2010target zooplankters. Adaptation of the fluorescence signal would greatly expedite traditional methods of enumerating salmon louse larvae in plankton samples and could provide a means of automated detection.<\/jats:p>","DOI":"10.1111\/jfd.13345","type":"journal-article","created":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T19:08:13Z","timestamp":1613588893000},"page":"863-879","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Illuminating the planktonic stages of salmon lice: A unique fluorescence signal for rapid identification of a rare copepod in zooplankton assemblages"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3318-4064","authenticated-orcid":false,"given":"Cameron R. S.","family":"Thompson","sequence":"first","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3544-0519","authenticated-orcid":false,"given":"James E.","family":"Bron","sequence":"additional","affiliation":[{"name":"University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7885-2989","authenticated-orcid":false,"given":"Samantha","family":"Bui","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Matre Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6092-4710","authenticated-orcid":false,"given":"Sussie","family":"Dalvin","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7899-5445","authenticated-orcid":false,"given":"Mark J.","family":"Fordyce","sequence":"additional","affiliation":[{"name":"Marine Scotland Science Aberdeen Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8577-9604","authenticated-orcid":false,"given":"Tomasz","family":"Furmanek","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1550-6007","authenticated-orcid":false,"given":"Gunnv\u00f8r","family":"\u00e1 Nor\u00f0i","sequence":"additional","affiliation":[{"name":"Fiskaaling \u2010 Aquaculture Research Station of the Faroes Hvalv\u00edk Faroe Islands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6983-8907","authenticated-orcid":false,"given":"Rasmus","family":"Skern\u2010Mauritzen","sequence":"additional","affiliation":[{"name":"Institute of Marine Research Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00134"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aqrep.2019.100216"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/691981"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/05704928.2012.692105"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13657305.2019.1654558"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1998.0232"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsy015"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2008.07.004"},{"key":"e_1_2_10_10_1","volume-title":"Finding the needle in a haystack: comparison of enumeration methods for a rare parasitic copepod species in plankton samples","author":"Bui","year":"2020"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.12.009"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2016.03.021"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4203(95)00062-3"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.01011.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2009.0771"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.04.004"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02331"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470875612.ch12"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3354\/ab00689"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/plankt\/19.4.449"},{"key":"e_1_2_10_21_1","volume-title":"Fisheries Global Information System (FIGIS)","author":"FAO","year":"2020"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00322"},{"key":"e_1_2_10_23_1","volume-title":"An improved method for the visualization of salmon louse Lepeophtheirus salmonis copepodids from plankton samples by fluorescence stereomicroscopy","author":"Fordyce M.","year":"2017"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/33.8.3894502"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps09045"},{"key":"e_1_2_10_26_1","volume-title":"Studies on the biology and ecology of the free swimming larval stages of Lepeophtheirus salmonis (Kroyer, 1838) and Caligus elongatus Nordmann, 1832 (Copepoda: Caligidae)","author":"Gravil H. R.","year":"1996"},{"issue":"6","key":"e_1_2_10_27_1","first-page":"1","article-title":"Zooplankton investigations in some fjords in Western Norway during 1950\u20131951","volume":"10","author":"Gundersen K. R.","year":"1953","journal-title":"Fiskeridirektoratets Skrifter"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073539"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2009.08.009"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2004.12.027"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/rra.1005"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.epidem.2017.11.001"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1148744"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2004.3027"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2012.2359"},{"key":"e_1_2_10_36_1","volume-title":"Principles of fluorescence spectroscopy","author":"Lakowicz J. R.","year":"2013"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3ay41160e"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201338"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00359"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12692"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.58.007022"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00227-002-0868-8"},{"key":"e_1_2_10_43_1","volume-title":"Sampling strategies, distribution and concentration of planktonic salmon lice copepods in the Outer Hardangerfjord and the Altafjord","author":"Nilsen M. K.","year":"2016"},{"key":"e_1_2_10_44_1","volume-title":"ICES zooplankton status report 2010\/2011","author":"O'Brien T. D.","year":"2013"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00022"},{"key":"e_1_2_10_46_1","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2018"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2010.01.012"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.17221\/687-CJFS"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12065"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjfas-2016-0050"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00193"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsz256"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0025315404008963h"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00342"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jembe.2020.151429"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-15-32"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps290263"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsu132"},{"key":"e_1_2_10_59_1","doi-asserted-by":"crossref","unstructured":"Thompson C. R. Bron J. Bui S. Dalvin S. Fordyce M. J. Furmanek T. \u00e1 Nor\u00f0i G. &Skern\u2010Mauritzen R.(2020).Illuminating the planktonic stages of salmon lice: a unique fluorescence signal for rapid identification of a rare copepod in zooplankton assemblages. [Data set].Zendo.https:\/\/doi.org\/10.5281\/zenodo.4157041","DOI":"10.1111\/jfd.13345"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7810"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00142"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2010.02.010"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12061"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsx104"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13345","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13345","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T23:06:05Z","timestamp":1693350365000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,15]]},"references-count":63,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1111\/jfd.13345"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13345","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,15]]},"assertion":[{"value":"2020-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}