{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:10:48Z","timestamp":1790424648849,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T00:00:00Z","timestamp":1615852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Tilapia tilapinevirus<\/jats:italic>\n                    \u00a0or tilapia lake virus (TiLV) is an emerging virus that inflicts significant mortality on farmed tilapia globally. Previous studies reported detection of the virus in multiple\u00a0organs of\u00a0the infected fish; however, little is known about the in\u2010depth localization of the virus in the central nervous system. Herein, we determined the\u00a0distribution of TiLV\u00a0in the entire brain of\u00a0experimentally\u00a0infected Nile tilapia. In situ\u00a0hybridization (ISH) using TiLV\u2010specific probes revealed that the virus was broadly distributed throughout the brain. The strongest positive signals were dominantly detected in the forebrain (responsible for learning, appetitive behaviour and attention) and the hindbrain (involved in controlling locomotion and basal physiology). The permissive cell zones for viral infection were observed mostly to be along the blood vessels and the ventricles. This indicates that the virus may productively enter into the brain through the circulatory system and widen broad regions, possibly through the cerebrospinal fluid along the ventricles, and subsequently induce the brain dysfunction. Understanding the pattern of viral localization in the brain may help elucidate the neurological\u00a0disorders\u00a0of the diseased fish.\u00a0This study revealed the distribution of TiLV in the whole infected brain, providing new insights into fish\u2013virus\u00a0interactions and neuropathogenesis.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13367","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T09:57:36Z","timestamp":1615888656000},"page":"1053-1064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Dissecting the localization of\n                    <i>Tilapia<\/i>\n                    <i>tilapinevirus<\/i>\n                    in the brain of the experimentally infected Nile tilapia,\n                    <i>Oreochromis<\/i>\n                    <i>niloticus<\/i>\n                    (L.)"],"prefix":"10.1111","volume":"44","author":[{"given":"Nguyen","family":"Dinh\u2010Hung","sequence":"first","affiliation":[{"name":"Fish Infectious Diseases Research Unit (FID RU) Department of Veterinary Microbiology Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"},{"name":"The International Graduate Course of Veterinary Science and Technology (VST) Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pattiya","family":"Sangpo","sequence":"additional","affiliation":[{"name":"Faculty of Science and Technology Suan Sunandha Rajabhat University Bangkok Thailand"},{"name":"Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanapong","family":"Kruangkum","sequence":"additional","affiliation":[{"name":"Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Bangkok Thailand"},{"name":"Department of Anatomy Faculty of Science Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6187-2772","authenticated-orcid":false,"given":"Pattanapon","family":"Kayansamruaj","sequence":"additional","affiliation":[{"name":"Center of Excellence in Aquatic Animal Health Management Faculty of Fisheries Kasetsart University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilladit","family":"Rung\u2010ruangkijkrai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy Faculty of Veterinary Science Chulalongkorn University Bangkok 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