{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T23:00:52Z","timestamp":1784242852572,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T00:00:00Z","timestamp":1618272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Between August and December 2013, the offshore cages of a commercial marine farm culturing red drum\n                    <jats:italic>Sciaenops ocellatus<\/jats:italic>\n                    in Campeche Bay Mexico were affected by an outbreak of an ulcerative granulomatous disease with up to 70% cumulative mortality. Thirty\u2010one adults displaying open ulcers on the skin were submitted for diagnosis. At necropsy, multiple white\u2010yellowish nodules (0.1\u20130.5\u00a0cm in diameter) were present in all internal organs, where the kidney and the spleen were the most severely affected. Histopathology evinced typical systemic granulomatous formations. Gram and Ziehl\u2013Neelsen stains on tissue imprints, bacterial swabs and tissue sections revealed Gram\u2010positive, acid\u2010fast, branching beaded long rod filamentous bacteria. Tissue samples resulted positive for nocardiosis with a\n                    <jats:italic>Nocardia<\/jats:italic>\n                    genus\u2010specific nested PCR. Definite identification at the species level and taxonomic positioning of the fastidious pathogen were achieved through a specific\n                    <jats:italic>Nocardia seriolae<\/jats:italic>\n                    PCR and by sequencing the\n                    <jats:italic>gyrB<\/jats:italic>\n                    gene of pure isolates. After administration of antibiotics during fry production, a posterior follow\u2010up monitoring (from 2014 to 2017) detected mild but recurrent outbreaks of the bacteria with no seasonality pattern. To the extent of our knowledge, this is the first report of piscine nocardiosis in Mexico and the first time this disease is detected in red drum.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13373","type":"journal-article","created":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T23:54:21Z","timestamp":1618358061000},"page":"1117-1130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["First evidence of fish nocardiosis in Mexico caused by\n                    <i>Nocardia seriolae<\/i>\n                    in farmed red drum (\n                    <i>Sciaenops ocellatus<\/i>\n                    , Linnaeus)"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9750-4226","authenticated-orcid":false,"given":"Rodolfo Enrique","family":"del Rio\u2010Rodriguez","sequence":"first","affiliation":[{"name":"Laboratorio de Sanidad Acu\u00edcola Instituto EPOMEX Campus 6 de Investigaciones Universidad Aut\u00f3noma de Campeche Campeche M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6260-5158","authenticated-orcid":false,"given":"Jose Gustavo","family":"Ramirez\u2010Paredes","sequence":"additional","affiliation":[{"name":"Ridgeway Biologicals Ltd. a Ceva Sant\u00e9 Animale Company Berkshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0760-4029","authenticated-orcid":false,"given":"Sonia Araceli","family":"Soto\u2010Rodriguez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Alimentaci\u00f3n y Desarrollo Unidad Mazatl\u00e1n en Acuicultura y Manejo Ambiental Mazatl\u00e1n Sinaloa M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yechiam","family":"Shapira","sequence":"additional","affiliation":[{"name":"Phibro Israel \u2013 Aquaculture Phibro Animal Health Corporation Yoqneam Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana del Jesus","family":"Huchin\u2010Cortes","sequence":"additional","affiliation":[{"name":"Laboratorio de Sanidad Acu\u00edcola Instituto EPOMEX Campus 6 de Investigaciones Universidad Aut\u00f3noma de Campeche Campeche M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Ruiz\u2010Hernandez","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda Facultad de Ciencias Qu\u00edmico\u2010Biol\u00f3gicas Campus 5 Universidad Aut\u00f3noma de Campeche Campeche M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica Isela","family":"Gomez\u2010Solano","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda Facultad de Ciencias Qu\u00edmico\u2010Biol\u00f3gicas Campus 5 Universidad Aut\u00f3noma de Campeche Campeche M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Haydon","sequence":"additional","affiliation":[{"name":"Ridgeway Biologicals Ltd. a Ceva Sant\u00e9 Animale Company Berkshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-7345.2012.00573.x"},{"key":"e_1_2_9_3_1","first-page":"735","article-title":"Mycobacterium marinum. Tuberculosis and Nontuberculous","author":"Aubry A.","year":"2017","journal-title":"Mycobacterial Infections"},{"key":"e_1_2_9_4_1","volume-title":"An aquaculture manual for Seriola lalandi (Pisces: Carangidae)","author":"Aviles\u2010Quevedo A.","year":"2004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2000.00201.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2011.01269.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12259"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12015"},{"key":"e_1_2_9_9_1","unstructured":"FAO(2005\u20102020).Cultured aquatic species information programme. Sciaenops ocellatus. Text by Cynthia K. Faulk A. In: FAO Fisheries Division [online]. Rome. Updated 9 February 2005. [Accessed 27 November 2020].http:\/\/www.fao.org\/fishery\/collection\/cultured\u2010species\/en"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118960608.gbm00842"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-008-9226-1"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.656"},{"key":"e_1_2_9_13_1","volume-title":"Kirby\u2010Bauer disk diffusion susceptibility test protocol","author":"Hudzicki J.","year":"2009"},{"key":"e_1_2_9_14_1","first-page":"25","article-title":"Hatchery production of pacific white snook at CIAD\u2010Unity Mazatlan, Mexico","volume":"25","author":"Ibarra\u2010Castro L.","year":"2017","journal-title":"World Aquaculture"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01606-15"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.02872.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2006.00748.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.09.006"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"issue":"3","key":"e_1_2_9_20_1","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Kumar T.","year":"1993","journal-title":"Molecular Biology and Evolution"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2331\/suisan.55.1183"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2331\/suisan.40.369"},{"key":"e_1_2_9_23_1","first-page":"297","volume-title":"Nocardial infections in fish: An emerging problem in both freshwater and marine aquaculture systems in Asia. Diseases in Asian aquaculture VI","author":"Labrie L.","year":"2008"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12782"},{"key":"e_1_2_9_25_1","first-page":"20","article-title":"Granulomatosis in fish aquaculture: A mini review","volume":"1","author":"Mart\u00ednez\u2010Lara P.","year":"2020","journal-title":"Reviews in Aquaculture"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.38.93"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652014000500005"},{"key":"e_1_2_9_28_1","first-page":"193","volume-title":"Development of vaccines against nocardiosis in fishes","author":"Nayak S. K.","year":"2016"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004009"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00970-17"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12372"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00120-X"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03023"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2005.00637.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.00991.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR2014.6874"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.697-703.1991"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935542.0000"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.3.1104-1109.1995"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173198"},{"key":"e_1_2_9_41_1","volume-title":"Marine Science assessment of capture\u2010based tuna (Thunnus orientalis) aquaculture in the Ensenada region of northern Baja California, Mexico","author":"Zertuche\u2010Gonz\u00e1lez J. A.","year":"2008"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13373","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T14:51:33Z","timestamp":1693320693000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,13]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1111\/jfd.13373"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13373","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.01.15.426713","asserted-by":"object"}]},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,13]]},"assertion":[{"value":"2021-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}