{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:25:18Z","timestamp":1783038318140,"version":"3.54.6"},"reference-count":66,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The structural response and plasticity of the cestode tegument in response to the influence of the host organism is not yet well understood. The main aims of our in vitro study were to analyse the ultrastructural mechanisms and kinetics of tegumental secretion in two cestode species, <jats:italic>Dibothriocephalus<\/jats:italic> <jats:italic>dendriticus<\/jats:italic> and <jats:italic>Ligula interrupta<\/jats:italic>, in response to the influence of fish host blood serum. The incubation of plerocercoids in the culture medium, which contained fish host blood serum, resulted in an increased number of secretory products on the tegumental surface. Our study is the first to experimentally demonstrate the formation of plerocercoid protective layers influenced by the host's internal environment factors. The mechanism of the generation of the protective layer included the following: the intensive formation of organelles in the tegumental cytons and their transfer to the distal cytoplasm of the tegument; increases in extracellular vesicles and vacuoles released on the tegumental surface; arrangement of secretory products and fine\u2010dispersed extracellular matrix in layers; and formation of the protective layer. The structural tegumental response included increases in the glycocalyx layer and structural changes. Our study revealed that the universal mechanism of protective layer formation was intrinsic to different tapeworms. We hypothesize that plerocercoids of cestodes parasitizing fish may use tegumental secretion in the formation of a protective layer and in the release of immunoregulator molecules to evade the host's immune response.<\/jats:p>","DOI":"10.1111\/jfd.13386","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T14:47:56Z","timestamp":1619707676000},"page":"1237-1254","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Experimental study of ultrastructural mechanisms and kinetics of tegumental secretion in cestodes parasitizing fish (Cestoda: Diphyllobothriidea)"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1119-6267","authenticated-orcid":false,"given":"Ivan Alexandrovich","family":"Kutyrev","sequence":"first","affiliation":[{"name":"Institute of General and Experimental Biology Siberian Branch of Russian Academy of Sciences Ulan\u2010Ude Russia"},{"name":"Institute of Biology Irkutsk State University Irkutsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5481-0207","authenticated-orcid":false,"given":"Natalia Mikhailovna","family":"Biserova","sequence":"additional","affiliation":[{"name":"Department of Invertebrate Zoology Faculty of Biology M.V. Lomonosov Moscow State University Moscow Russia"},{"name":"Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2676-3252","authenticated-orcid":false,"given":"Olga Evgenievna","family":"Mazur","sequence":"additional","affiliation":[{"name":"Institute of General and Experimental Biology Siberian Branch of Russian Academy of Sciences Ulan\u2010Ude Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhargal Nimaevich","family":"Dugarov","sequence":"additional","affiliation":[{"name":"Institute of General and Experimental Biology Siberian Branch of Russian Academy of Sciences Ulan\u2010Ude Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.07.013"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2017.05.003"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00418519"},{"key":"e_1_2_10_5_1","unstructured":"Avtandilov G. G.(1990).Medical morphometry. Handbook. \u2013 Moscow: Medicine."},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/ap-2016-0058"},{"key":"e_1_2_10_7_1","volume-title":"Methods of visualization for biological ultrastructures","author":"Biserova N. M.","year":"2013"},{"key":"e_1_2_10_8_1","first-page":"13","volume-title":"The problems of cestodology","author":"Biserova N. M.","year":"2012"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0012496611060019"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008557"},{"key":"e_1_2_10_11_1","first-page":"54","article-title":"Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity","volume":"25","author":"Buck A. H.","year":"2014","journal-title":"Nature Communications"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2016.06.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2015.06.009"},{"key":"e_1_2_10_14_1","first-page":"211","volume-title":"Microscopic anatomy of the invertebrates, Vol. 3. Platyhelminthes and Nemertea","author":"Coil W. H.","year":"1991"},{"key":"e_1_2_10_15_1","first-page":"32","article-title":"The morphology of two types of frontal glands of Grillotia erinaceus (Cestoda: Trypanorhyncha)","volume":"1","author":"Davydov V. G.","year":"1985","journal-title":"Parazitologiia"},{"key":"e_1_2_10_16_1","first-page":"177","volume-title":"Physiology and parasitology of freshwater animals. Proceeding of the Institute of Inner Waters","author":"Davydov V. G.","year":"1979"},{"key":"e_1_2_10_17_1","first-page":"88","volume-title":"Immunological and biochemical aspects of parasite\u2010host interactions. Proceeding of helminthological laboratory","author":"Davydov V. G.","year":"1988"},{"key":"e_1_2_10_18_1","first-page":"449","article-title":"Functional morphology of frontal and uterine glands in cestodes of the order Caryophyllidea","volume":"6","author":"Davydov V. G.","year":"1988","journal-title":"Parazitologiya"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2007.00833.x"},{"key":"e_1_2_10_20_1","volume-title":"Protozoan and metazoan infection","author":"Dick T. A.","year":"1995"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02707"},{"key":"e_1_2_10_22_1","volume-title":"Tapeworms (Cestoda, Ligulidae) of the fauna of the USSR","author":"Dubinina M. N.","year":"1980"},{"key":"e_1_2_10_23_1","first-page":"781","volume-title":"Molecular detection of foodborne pathogens","author":"Dupouy\u2010Camet J.","year":"2010"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-015-4659-9"},{"key":"e_1_2_10_25_1","first-page":"212","article-title":"Structural response of the cestode tegument to the host blood serum in incubation experiments","volume":"65","author":"Golovaneva M. S.","year":"2019","journal-title":"Annals of Parasitology"},{"key":"e_1_2_10_26_1","first-page":"13","volume-title":"The early brain","author":"Gustafsson M. K. S.","year":"1990"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2014.03.007"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.04.008"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182010000107"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601996"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2001)087[0055:COTLLO]2.0.CO;2"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00177-0"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00055-1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11686-020-00318-8"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.08.005"},{"key":"e_1_2_10_36_1","volume-title":"Functional morphology of the lower cestodes: Ontogenetic and evolutionary aspects","author":"Kuperman B. I.","year":"1988"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(82)90031-5"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(82)90008-X"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2017.01.006"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.09.031"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.01.022"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1164-y"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2004.09.006"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1183"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022017000401482"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045974"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-48996-2"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02487"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.15298\/invertzool.14.2.09"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007032"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1134\/S2079086417020050"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1092"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931051"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2003.047"},{"key":"e_1_2_10_55_1","first-page":"325","article-title":"Frontal glands in the pseudoscolex of Paraechinophallus japonicus (Yamaguti, 1934) (Cestoda, Bothriocephalidea, Echinophallidae)","volume":"4","author":"Poddubnaya L. G.","year":"2007","journal-title":"Acta Parasitologica"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.08.029"},{"key":"e_1_2_10_57_1","volume-title":"Evolutionary parasitology: The integrated study of infections, immunology, ecology, and genetics","author":"Schmid\u2010Hempel P.","year":"2011"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8030(96)90012-5"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1989.tb03029.x"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2519-4"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02672"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2010.010048"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2018.08.002"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2017.06.004"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/ivb.12202"},{"key":"e_1_2_10_66_1","first-page":"139","article-title":"Ultrastructure of three types of scolex gland cells in adult Bothriocephalus claviceps (Cestoda: Pseudophyllidea)","volume":"44","author":"\u017dd\u2019\u00e1rsk\u00e1 Z.","year":"1997","journal-title":"Folia Parasitologica"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2008.01840.x"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13386","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13386","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13386","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T18:52:04Z","timestamp":1693335124000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,29]]},"references-count":66,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1111\/jfd.13386"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13386","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,29]]},"assertion":[{"value":"2021-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}