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To date, there has no report regarding the ability of this virus to infect freshwater fish. In this study, we screened and discovered CMNV\u2010positive freshwater zebrafish individuals by reverse transcription\u2010nested PCR (RT\u2010nPCR). The sequence of CMNV amplicons from zebrafish was found to share 99% identity with RNA\u2010dependent RNA polymerase (RdRp) gene of the original CMNV isolate. Histopathological examination of the CMNV\u2010positive zebrafish samples revealed extensive vacuolation and karyopyknosis lesions in the retina of the eye and the midbrain mesencephalon. CMNV\u2010like virus particles were visualized in these tissues under transmission electron microscope. Different degrees of pathological damages were also found in muscle, gills, thymus and ovarian tissues. Strong positive signals of CMNV probe were observed in these infected tissues by <jats:italic>in situ<\/jats:italic> hybridization. Overall, all results indicated that zebrafish, an acknowledged model organism, could be infected naturally by CMNV. Thus, it is needed to pay close attention to the possible interference of CMNV whether in assessment of toxic substances, or in studying the developmental characterization and the nerval function, when zebrafish was used as model animal.<\/jats:p>","DOI":"10.1111\/jfd.13390","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T09:26:10Z","timestamp":1623230770000},"page":"1315-1324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Natural infection of covert mortality nodavirus affects Zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1111","volume":"44","author":[{"given":"Chong","family":"Wang","sequence":"first","affiliation":[{"name":"Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Maricultural Organism Disease Control Ministry of Agriculture Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[{"name":"Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Maricultural Organism Disease Control Ministry of Agriculture Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy F. 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