{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:09:02Z","timestamp":1791947342266,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007280","name":"Srinakharinwirot University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (<jats:italic>Lates calcarifer<\/jats:italic>). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil\u2010DNA glycosylase (UDG)\u2010supplemented cross\u2010priming amplification (UCPA) combined with a colorimetric detection method using the hydroxynaphthol blue (HNB) and lateral flow dipstick (LFD) for detection of SDDV was developed. The UDG was utilized to prevent carryover contamination, and the CPA reactions can be readily observed by HNB and LFD. The CPA primers and probe were designed to target the major capsid protein (<jats:italic>MCP<\/jats:italic>) gene of the SDDV. The optimized UCPA conditions were performed at the temperature of 61\u00b0C for 60\u00a0min. The UCPA assays demonstrated specificity to SDDV without cross\u2010reaction to other tested viruses including red\u2010spotted grouper nervous necrosis virus (RGNNV), infectious spleen and kidney necrosis virus (ISKNV) and <jats:italic>Lates calcarifer<\/jats:italic> herpes virus (LCHV), and other bacterial species commonly found in aquatic animals. The sensitivity of the UCPA\u2010HNB and UCPA\u2010LFD was 100 viral copies\/\u00b5l and 10\u00a0pg of extracted total DNA, which was 10\u2010fold more sensitive than that of conventional PCR. The UCPA\u2010HNB and UCPA\u2010LFD assays could be used to detect the SDDV infection in all 25 confirmed SDDV\u2010infected fish samples. Therefore, the UCPA coupled with HNB and LFD was rapid, simple and effective and might be applied for diagnosis of SDDV infection.<\/jats:p>","DOI":"10.1111\/jfd.13448","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T07:54:57Z","timestamp":1622102097000},"page":"1411-1422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development of cross\u2010priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection"],"prefix":"10.1111","volume":"44","author":[{"given":"Terawut","family":"Prasitporn","sequence":"first","affiliation":[{"name":"Department of Biology Faculty of Science Srinakharinwirot University Bangkok Thailand"},{"name":"Center of Excellence in Animal, Plant and Parasite Biotechnology Srinakharinwirot University Bangkok 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