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Bcg includes several closely related species, two of which, <jats:italic>B<\/jats:italic>. <jats:italic>cereus<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>thuringiensis<\/jats:italic>, are pathogens of aquatic animals or insects. In the present study, we collected 57 Bcg isolates from diseased CST from 2016 to 2019 in Kaohsiung and Pingtung, where most CST farms are located in Taiwan. All isolates were divided into four genotypes with two restriction enzymes, <jats:italic>Sma<\/jats:italic>I and <jats:italic>Not<\/jats:italic>I, by pulsed\u2010field gel electrophoresis and enterobacterial repetitive intergenic consensus\u2013polymerase chain reaction (ERIC\u2010PCR). Representative isolates from each genotype were subjected to phylogenetic tree analysis using 16S rDNA and pyruvate carboxylase genes as phylogenetic markers, and these CST isolates appeared in different clades. PCR was performed targeting six selected virulence genes, four of which were detected in CST isolates, including cytotoxin K (1\/57), <jats:italic>hblC<\/jats:italic> of the haemolysin BL complex (46\/57), <jats:italic>nheA<\/jats:italic> of the non\u2010haemolytic enterotoxin complex (52\/57) and enterotoxin FM (57\/57), whereas cereulide synthetase and cereulide peptide synthase\u2010like genes were not detected in any isolates.<\/jats:p>","DOI":"10.1111\/jfd.13473","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T14:47:28Z","timestamp":1623682048000},"page":"1515-1529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Genotype and virulence gene analyses of <i>Bacillus cereus<\/i> group clinical isolates from the Chinese softshell turtle (<i>Pelodiscus sinensis<\/i>) in 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