{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T22:37:25Z","timestamp":1788820645379,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T00:00:00Z","timestamp":1629849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Pseudomonas plecoglossicida<\/jats:italic>, the causative agent of visceral granulomas in the large yellow croaker (<jats:italic>Larimichthys crocea<\/jats:italic>) in China, encodes three sets of type \u2165 secretion systems (T6SS1\u20103). The purpose of this study was to characterize the different roles of T6SSs involved in infection. In\u2010frame deletion of T6SSs was constructed, which resulted in 8 mutants. Competition against <jats:italic>E.\u00a0coli<\/jats:italic> DH5\u03b1, virulence against the croaker and in vivo survival ability of the mutants were tested. The expression and secretion of Hcp by <jats:italic>P.\u00a0plecoglossicida<\/jats:italic> NB2011 were investigated. The results showed T6SS2 mutant failed to inhibit the growth of <jats:italic>E.\u00a0coli<\/jats:italic>, which is an indication of T6SS2 acting against environmental bacteria. The LD<jats:sub>50<\/jats:sub> value of T6SS1 mutant strongly increased; T6SS2 and T6SS3 mutants were similar to that of the wild type; and the virulence of double deletion or triple deletion mutant was drastically alleviated, indicating that T6SS1 being one of the major virulence factors, and T6SS2 and T6SS3 directly or indirectly being involved in the pathogenicity. T6SS1 mutant disappeared in the fish spleen in 3\u00a0days, while other strains kept increasing, indicating the T6SS1 stimulation bacteria replication in vivo. Hcp1 secreted at 12\u201328\u00b0C and Hcp2 secreted at 12\u201335\u00b0C, while Hcp3 secretion not detected in vitro. This study has thrown some insights on the understanding of pathogenicity mechanisms of this pathogen.<\/jats:p>","DOI":"10.1111\/jfd.13479","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T08:52:07Z","timestamp":1629881527000},"page":"1669-1679","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Preliminary studies on the different roles of T6SSs in pathogenicity of <i>Pseudomonas plecoglossicida<\/i> NB2011"],"prefix":"10.1111","volume":"44","author":[{"given":"Jiamin","family":"Jin","sequence":"first","affiliation":[{"name":"Biological and Environmental College Zhejiang Wanli University Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Li","sequence":"additional","affiliation":[{"name":"Biological and Environmental College Zhejiang Wanli University Ningbo 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