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Glycoprotein (G), as the most important antigenic determinant protein of SVCV, is widely considered as an effective method against SVCV. In our previous study, we found that G3 (131 aa) is the potential dominant antigen epitope that induces strong immune responses similar to G protein (510 aa). Here, in order to further improve the immune effect, we reported a subunit vaccine (PEG\u2010G3) constructed by PEG\u2010modified dominant epitope protein (G3). The results of serum antibody production, enzyme activities and immune\u2010related genes expression showed that PEG\u2010G3 induces significantly stronger immune protective responses against SVCV than G3. PEG modification significantly increased the serum antibody level of the vaccine, which increased significantly after immunization and reached the peak at 21\u00a0day post\u2010vaccination. T\u2010AOC and AKP activities in the lowest concentration group (5\u00a0\u03bcg) of PEG\u2010G3 were significantly higher than those in the highest concentration group (20\u00a0\u03bcg) of G3. In PEG\u2010G3 group, the expression of almost all genes increased at least 4 times compared with the control group. After 14\u2010day challenge, the RPS (relative percentage survival) of the highest concentration of PEG\u2010G3 group was 53.6%, while that of G3 group is 38.9%. Therefore, this work shows that PEG modification and dominant epitope screening may be effective methods to improve the immune protective effect of vaccines and to resist the infection of aquatic animal viral diseases.<\/jats:p>","DOI":"10.1111\/jfd.13481","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T15:57:12Z","timestamp":1624550232000},"page":"1587-1594","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["PEG\u2010modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus"],"prefix":"10.1111","volume":"44","author":[{"given":"Hui\u2010Xin","family":"Duan","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling 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