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Despite its significance on fish function and welfare, normal skeletal development has rarely been evaluated in establishing the best feeding practices for zebrafish. The aim of this study was to establish a protocol for normal skeletal development, growth and survival of zebrafish larvae through live feed\u2010to\u2010microdiet transition at an appropriate rate. Four feeding regimes including feeding exclusively on <jats:italic>Artemia<\/jats:italic> nauplii (A) or dry microdiet (D), and feeding on both <jats:italic>Artemia<\/jats:italic> and microdiet at two different transition rates (slow (B) or fast (C)) were applied from 5 to 24 dpf (days post\u2010fertilization). Results demonstrated a significant effect of feeding regimes on the incidence of skeletal abnormalities (gill cover, fins and vertebral column, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in zebrafish larvae. The A and B experimental groups presented the highest (88\u00a0\u00b1\u00a03 and 84\u00a0\u00b1\u00a017%, respectively), but the C and D the lowest (18\u00a0\u00b1\u00a014 and 11\u00a0\u00b1\u00a02%, respectively), rates of normal fish (fish without any abnormality). Similarly, growth rate was comparatively elevated in A and B groups. No significant differences were observed in fish survival between A, B and C groups. However, D group presented a significantly lower survival rate. To our knowledge, this is the first study to show that the live feed\u2010to\u2010microdiet transition rate influences larval growth, survival and abnormality rates in a non\u2010homogenous pattern.<\/jats:p>","DOI":"10.1111\/jfd.13487","type":"journal-article","created":{"date-parts":[[2021,7,18]],"date-time":"2021-07-18T07:34:35Z","timestamp":1626593675000},"page":"1689-1696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Balancing between <i>Artemia<\/i> and microdiet usage for normal skeletal development in zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1111","volume":"44","author":[{"given":"Alice","family":"Printzi","sequence":"first","affiliation":[{"name":"Biology Department University of Crete Heraklion Greece"},{"name":"IFREMER Univ Brest CNRS IRD LEMAR Plouzan\u00e9 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