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To promote precise quantification of infection, the counting of black spots has to be confirmed by reliable quantification of metacercariae after tissue digestion. This process ensures the isolation of undamaged parasites for morphological and molecular identification. The aim of this work was to optimize the pepsin digestion protocol and to assess the duration of viability of <jats:italic>Cryptocotyle<\/jats:italic> metacercariae in fish post\u2010mortem (pm). Four digestion protocols were compared by measuring the viability rate of metacercariae. The present study shows that the orbital digestion method was the least destructive for metacercariae and allowed better quantification of <jats:italic>Cryptocotyle<\/jats:italic> infection. Moreover, morphological identification seemed reliable up to 8\u00a0days\u00a0pm for <jats:italic>Cryptocotyle<\/jats:italic> infection.<\/jats:p>","DOI":"10.1111\/jfd.13495","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T17:01:04Z","timestamp":1626886864000},"page":"1777-1784","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Optimization of tools for the detection and identification of <i>Cryptocotyle<\/i> metacercariae in fish: Digestion method and viability studies"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0376-7744","authenticated-orcid":false,"given":"Maureen","family":"Duflot","sequence":"first","affiliation":[{"name":"ANSES, Laboratory for Food Safety  Boulogne\u2010sur\u2010Mer France"},{"name":"University of Littoral C\u00f4te d'Opale  Boulogne\u2010sur\u2010Mer France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5336-0323","authenticated-orcid":false,"given":"Graziella","family":"Midelet","sequence":"additional","affiliation":[{"name":"ANSES, Laboratory for Food Safety  Boulogne\u2010sur\u2010Mer France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odile","family":"Bourgau","sequence":"additional","affiliation":[{"name":"ANSES, Laboratory for Food Safety  Boulogne\u2010sur\u2010Mer France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2747-237X","authenticated-orcid":false,"given":"Kurt","family":"Buchmann","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4644-5270","authenticated-orcid":false,"given":"M\u00e9lanie","family":"Gay","sequence":"additional","affiliation":[{"name":"ANSES, Laboratory for Food Safety  Boulogne\u2010sur\u2010Mer France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639\u2010020\u201001259\u20109"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2014.09.021"},{"key":"e_1_2_8_4_1","volume-title":"An introduction to fish parasitological methods\u2010classical and molecular techniques","author":"Buchmann K.","year":"2007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1645\/18\u201044"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-378612-8.00156-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.013"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2017.09.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.105100"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2016.54.4.537"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2307\/3224182"},{"key":"e_1_2_8_12_1","unstructured":"Codelite Ltd. 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