{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T10:04:53Z","timestamp":1791453893316,"version":"4.3.3"},"reference-count":82,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T00:00:00Z","timestamp":1626825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eye flukes in fish are common in freshwater lakes. Fish become infected by the penetration of cercariae released from freshwater snails, and high infection pressures may be associated with mortalities in a Danish lake. Examination of two other freshwater lakes, combined with laboratory study, supported the notion. We investigated 77 freshwater fish from two lakes and the infection level suggested the occurrence of a high cercarial infection pressure in the Danish lakes. Dominant genera were <jats:italic>Tylodelphys<\/jats:italic> and <jats:italic>Diplostomum<\/jats:italic> covering a range of species identified by PCR and sequencing of the <jats:italic>18S<\/jats:italic> (partial)\u2010<jats:italic>ITS1\u20105.8S\u2010ITS2<\/jats:italic>\u2010<jats:italic>28S<\/jats:italic> (partial) of the rDNA. Cercariae of the prevalent species <jats:italic>Diplostomum pseudospathaceum<\/jats:italic> were used to infect zebrafish <jats:italic>Danio rerio<\/jats:italic> for the elucidation of short\u2010term effects on the fish host. Zebrafish did not display abnormal behaviour when exposed to 200\u2013400 cercariae, but a dosage of 600 and 1,000 cercariae\/fish proved lethal. When fish were exposed to sublethal dosages, 19 out of 27 immune genes were significantly regulated and three genes encoding cytokine (IL 4\/13B, IL\u20106 and IL\u20108) were upregulated at 3\u00a0hr post\u2010infection (hpi), whereas others were downregulated especially at a later time point. We suggest that direct massive cercarial penetration of fish surfaces may be detrimental and may represent a threat to fish populations.<\/jats:p>","DOI":"10.1111\/jfd.13496","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T17:10:04Z","timestamp":1626887404000},"page":"1785-1798","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Eye fluke effects on Danish freshwater fish: Field and experimental investigations"],"prefix":"10.1111","volume":"44","author":[{"given":"Yajiao","family":"Duan","sequence":"first","affiliation":[{"name":"Laboratory of Aquatic Pathobiology Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6005-3249","authenticated-orcid":false,"given":"Louise","family":"von Gersdorff J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Pathobiology Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per Walter","family":"Kania","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Pathobiology Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma M.","family":"Karami","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Pathobiology Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azmi","family":"Al\u2010Jubury","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Pathobiology Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2747-237X","authenticated-orcid":false,"given":"Kurt","family":"Buchmann","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Pathobiology Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,21]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.008"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000372"},{"key":"e_1_2_12_4_1","volume-title":"An introduction to fish parasitological methods: Classical and molecular techniques","author":"Buchmann K.","year":"2007"},{"issue":"5","key":"e_1_2_12_5_1","first-page":"165","article-title":"Different seasonal infection dynamics of two species of eye flukes (Diplostomum spathaceum and Tylodelphys clavata) in rainbow trout (Oncorhynchus mykiss)","volume":"17","author":"Buchmann K.","year":"1997","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"issue":"3","key":"e_1_2_12_6_1","first-page":"104","article-title":"Effects of eyefluke infections on growth of rainbow trout (Oncorhynchus mykiss) in a mariculture system","volume":"14","author":"Buchmann K.","year":"1994","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284227"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010015\u20104830\u20103"},{"issue":"2","key":"e_1_2_12_9_1","first-page":"1","article-title":"Induction of pro\u2010inflammatory cytokines (IL\u20101 and IL\u20106) and lung inflammation by Coronavirus\u201019 (COVID\u201019 or SARS\u2010CoV\u20102): Anti\u2010inflammatory strategies","volume":"34","author":"Conti P.","year":"2020","journal-title":"Journal of Biological Regulators & Homeostatic Agents"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.07.002"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010020\u201004536\u2010x"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.2478\/s10086\u2010008\u20100006\u20104"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000026810"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010017\u20105439\u20105"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1139\/z02\u2010198"},{"key":"e_1_2_12_16_1","volume-title":"The freshwater gastropods of the West\u2010Palaearctis","author":"Gl\u00f6er P.","year":"2019"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00265\u2010015\u20101984\u2010z"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00265\u2010017\u20102300\u2010x"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.2005.B1045"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2013.09.011"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108001"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2015.09.008"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2016.05.005"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.34.1.41"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.86.717"},{"key":"e_1_2_12_26_1","unstructured":"Jensen H. J.(2015).Utterslev Mose. Environmental condition 2015. Fish Ecological Laboratory. Report to the Municipality of Copenhagen November 2015. 22 pp. (in Danish)."},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182005008681"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004005281"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182003003561"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010006\u20100137\u20108"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao01918"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004006006"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2015.06.009"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.2307\/2265536"},{"issue":"1","key":"e_1_2_12_36_1","first-page":"20","article-title":"Pathogenicity of Diplostomum cercariae in rainbow trout, and alternative measures to prevent diplostomosis in fish farms","volume":"25","author":"Larsen A.","year":"2005","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.2307\/3281395"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360100462"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao025169"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjz\u201079\u20103\u2010355"},{"issue":"3","key":"e_1_2_12_41_1","first-page":"429","article-title":"A sight for sore eyes: Diplostomum and Tylodelphys in the eyes of fish","volume":"11","author":"McCloughlin T.","year":"2016","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)80412\u20102"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.12363"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2004271"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X19000506"},{"issue":"2","key":"e_1_2_12_46_1","first-page":"89","article-title":"Parasite communities of European perch, Perca fluviatilis L. (Actinopterygii: Perciformes: Percidae) from Lake Lebsko (Central Coast, Poland","volume":"59","author":"Morozinska\u2010Gogol J.","year":"2013","journal-title":"Annals of Parasitology"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010019\u201006389\u20105"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010017\u20105564\u20101"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13028\u2010017\u20100343\u20107"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00010306"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020\u20107519(95)00106\u20109"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251\u2010008\u20100299\u2010x"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1577\/M05\u2010038.1"},{"key":"e_1_2_12_54_1","volume-title":"Parasitic diseases of fish","author":"Pike A.","year":"1994"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065\u20103454(10)41005\u20100"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20102011(92)90032\u2010Y"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp045"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20103305\u20107\u2010109"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2003.10.021"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2005.01.020"},{"issue":"5","key":"e_1_2_12_61_1","first-page":"871","article-title":"Host manipulation by parasites and risk of non\u2010host predation: Is manipulation costly in an eye fluke\u2013fish interaction?","volume":"8","author":"Sepp\u00e4l\u00e4 O.","year":"2006","journal-title":"Evolutionary Ecology Research"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X17001237"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.04.010"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2003.12.005"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2008.03.018"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.05.011"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1080\/13921657.1998.10541437"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201012\u2010141"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.05.016"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01685449"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169\u20104758(91)90022\u2010G"},{"issue":"2","key":"e_1_2_12_72_1","first-page":"147","article-title":"A new method of DNA extraction from the ethanol\u2010fixed parasitic worms","volume":"44","author":"Tkach V.","year":"1999","journal-title":"Acta Parasitologica"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2010.02.003"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432\u20101327.1999.00657.x"},{"key":"e_1_2_12_75_1","first-page":"47","article-title":"Aspects of the biology of diplostomid metacercarial (Digenea) populations occurring in fishes in different localities of northern Finland","author":"Valtonen E. T.","year":"1997","journal-title":"Finnish Zoological and Botanical Publishing Board"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169\u20104758(90)90349\u20109"},{"key":"e_1_2_12_77_1","volume-title":"Trematodes modulate aquatic food webs by altering host feeding behaviour","author":"Vivas Mu\u00f1oz J. C.","year":"2019"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7295"},{"key":"e_1_2_12_79_1","first-page":"433","article-title":"On the occurrence of developmental stages of blood parasitizing flukes in Danish freshwaters","volume":"16","author":"Wesenberg\u2010Lund C.","year":"1932","journal-title":"Naturens Verden"},{"key":"e_1_2_12_80_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X0001066X"},{"key":"e_1_2_12_81_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.1.51.pe55"},{"key":"e_1_2_12_82_1","unstructured":"Wisniewski W. L.(1958).The development cycle ofPosthodiplostomum brevicaudatum(v. Nordmann 1832) Kozicka Acta Parasitologica Polonica.6(8\/21)."},{"key":"e_1_2_12_83_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology5020023"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13496","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13496","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13496","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T13:22:02Z","timestamp":1692451322000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,21]]},"references-count":82,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1111\/jfd.13496"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13496","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,21]]},"assertion":[{"value":"2021-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}