{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T16:41:53Z","timestamp":1782319313552,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T00:00:00Z","timestamp":1629331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u2018Big belly\u2019 disease is a chronic, granulomatous bacterial enteritis and peritonitis, first reported in 3\u2010 to 4\u2010week\u2010old Asian seabass or barramundi, <jats:italic>Lates calcarifer<\/jats:italic> Bloch fry. Affected fry are emaciated and have a swollen abdomen, and the condition is referred to as \u2018skinny pot\u2010belly\u2019 or \u2018big belly\u2019 disease. In this study, histopathological examinations of diseased fish from a batch of 2\u2010month\u2010old, 6\u2010 to 8\u2010cm <jats:italic>L.\u00a0calcarifer<\/jats:italic> fingerlings, kept in seawater recirculating aquaculture systems, showed pathology resembling \u2018big belly\u2019 disease. Ethanol\u2010fixed tissues were tested positive using specific PCR primers based on 16S rRNA genes. In situ hybridization using dioxygenin\u2010labelled positive PCR products on formalin\u2010fixed paraffin\u2010embedded tissues showed positive reactions with intralesional, clusters of the large, \u2018big belly\u2019 coccobacilli. A phylogenetic tree constructed based on analyses of these 16S rRNA gene PCR products from five positive fish suggests that the \u2018big belly\u2019 bacterium is most likely a novel <jats:italic>Vibrio<\/jats:italic> species.<\/jats:p>","DOI":"10.1111\/jfd.13512","type":"journal-article","created":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T19:07:01Z","timestamp":1629400021000},"page":"1985-1992","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["PCR, in\u2010situ hybridization, and phylogenetic analysis suggest that \u2018big belly\u2019 disease in barramundi, <i>Lates calcarifer<\/i> (Bloch), is caused by a novel <i>Vibrio<\/i> species"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4673-6067","authenticated-orcid":false,"given":"Susan","family":"Gibson\u2010Kueh","sequence":"first","affiliation":[{"name":"Tropical Futures Institute James Cook University Singapore City Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celestine","family":"Terence","sequence":"additional","affiliation":[{"name":"Tropical Futures Institute James Cook University Singapore City Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian Zhe","family":"Chew","sequence":"additional","affiliation":[{"name":"James Cook University Singapore City Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph Angelo","family":"Uichanco","sequence":"additional","affiliation":[{"name":"James Cook University Singapore City Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Shen","sequence":"additional","affiliation":[{"name":"Tropical Futures Institute James Cook University Singapore City Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.736848"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472\u2010765X.2006.01989.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03281"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2001.00308.x"},{"key":"e_1_2_7_6_1","volume-title":"Diseases of Asian seabass (or barramundi), Lates calcarifer Bloch","author":"Gibson\u2010Kueh S.","year":"2012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2004.00594.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2004.00589.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.05.004"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12533"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2019.1621648"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439\u20100426.2003.00356.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:WIBI.0000040402.44340.0e"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.09.005"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12141"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/anu.12929"},{"key":"e_1_2_7_19_1","first-page":"77","volume-title":"Biology and culture of Asian seabass Lates calcarifer","author":"Russell D. 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