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Insufficient information exists on the molecular immune response of silver pomfret to AO infestation, so herein we simulated the process of silver pomfret being infected by AO. Translucent trophosomes were observed on the gills of AO\u2010infected fish. Transcriptome profiling was performed to investigate the effects of AO infection on the gill, kidney complex and spleen. Overall, 404,412,298 clean reads were obtained, assembling into 96,341 unigenes, which were annotated against public databases. In total, 2730 differentially expressed genes were detected, and few energy\u2010 and immune\u2010related genes were further assessed using RT\u2010qPCR. Moreover, activities of three immune\u2010related (SOD, AKP and ACP) and three energy\u2010related (PKM, LDH and GCK) enzymes were determined. AO infection activated the immune system and increased interleukin\u20101 beta and immunoglobulin M heavy chain levels. Besides, the PPAR signalling pathway was highly enriched, which played a role in improving immunity and maintaining homeostasis. AO infection also caused dyspnoea, leading to extensive lactic acid accumulation, potentially contributing towards a strong immune response in the host. Our data improved our understanding regarding the immune response mechanisms through which fish coped with parasitic infections and may help prevent high fish mortality in aquaculture.<\/jats:p>","DOI":"10.1111\/jfd.13524","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T08:03:55Z","timestamp":1632902635000},"page":"2111-2123","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Immune response of silver pomfret (<i>Pampus argenteus<\/i>) to <i>Amyloodinium ocellatum<\/i> infection"],"prefix":"10.1111","volume":"44","author":[{"given":"Youyi","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Marine Biotechnology Ministry of Education Ningbo University Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University Ningbo China"},{"name":"College of marine Sciences Ningbo University Ningbo 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