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Specific pathogens can interfere with the experimental results and in the life quality of the animals. <jats:italic>Pseudoloma neurophilia<\/jats:italic> is a parasite with high potential for interference in behavioural, morphology, toxicological and genetic research, and is very common in zebrafish facilities. With that, we implemented a protocol for the pathogen elimination in a genetically modified lineage (<jats:italic>prop 1<\/jats:italic>) using eggs from specific pathogen\u2010free (SPF) wild\u2010type fish (AB line) for in vitro fertilization, along with water recirculation equipment disinfection, appropriate PCR screening and back crossing protocols. This resulted in SPF <jats:italic>prop 1<\/jats:italic> heterozygotes, which allowed us to move forward with subsequent crossings to develop homozygote <jats:italic>prop 1<\/jats:italic> mutants for our research. Hence, this demonstrates a useful strategy for an individual research laboratory to rederive a specific mutant free line that is not available from other SPF laboratories.<\/jats:p>","DOI":"10.1111\/jfd.13529","type":"journal-article","created":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T18:42:30Z","timestamp":1631731350000},"page":"35-39","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rederivation of a mutant line (<i>prop 1<\/i>) of zebrafish <i>Danio rerio<\/i> infected with <i>Pseudoloma neurophilia<\/i> using in vitro fertilization with eggs from pathogen\u2010free wild\u2010type (AB) females and sperm from <i>prop 1<\/i> males"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2213-4355","authenticated-orcid":false,"given":"Bianca H.","family":"Ventura Fernandes","sequence":"first","affiliation":[{"name":"Centro de Bioterismo da Faculdade de Medicina FMUSP Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Endocrinology discipline internal medicine department Hospital das Clinicas HCFMUSP Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9044-7348","authenticated-orcid":false,"given":"Caroline","family":"Caetano da Silva","sequence":"additional","affiliation":[{"name":"Endocrinology discipline internal medicine department Hospital das Clinicas HCFMUSP Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Inserm U1132 and Universit\u00e9 de Paris Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3084-6248","authenticated-orcid":false,"given":"Debora","family":"Bissegato","sequence":"additional","affiliation":[{"name":"Centro de Bioterismo da Faculdade de Medicina FMUSP Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7968-5142","authenticated-orcid":false,"given":"Michael L.","family":"Kent","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Biomedical Sciences Oregon State University Corvallis, OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7313-2130","authenticated-orcid":false,"given":"Luciani R.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Centro de Bioterismo da Faculdade de Medicina FMUSP Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Endocrinology discipline internal medicine department Hospital das Clinicas HCFMUSP Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,15]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.3354\/dao029213"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1089\/zeb.2018.1696"},{"key":"e_1_2_7_4_1","first-page":"339","article-title":"Study of transovarian transmission of mycobacteria in Betta splendens Regan","volume":"3","author":"Chinabut S.","year":"1994","journal-title":"Proceed 3rd Asian Fish"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.20945\/2359\u20103997000000139"},{"issue":"4","key":"e_1_2_7_6_1","first-page":"412","article-title":"Comparison of antemortem and environmental samples for zebrafish health monitoring and quarantine","volume":"56","author":"Crim M. 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