{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:41:08Z","timestamp":1785739268886,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T00:00:00Z","timestamp":1632700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000200","name":"United States Agency for International Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000200","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sixteen countries, including Bangladesh, have reported the presence of tilapia lake virus (TiLV), an emerging tilapia pathogen. Fish polyculture is a common farming practice in Bangladesh. Some unusual mortalities reported in species co\u2010cultivated with TiLV\u2010infected tilapia led us to investigate whether any of the co\u2010cultivated species would also test positive for TiLV and whether they were susceptible to TiLV infection under controlled laboratory experiments. Using 183 samples obtained from 15 farms in six districts across Bangladesh, we determined that 20% of the farms tested positive for TiLV in tilapia, while 15 co\u2010cultivated fish species and seven other invertebrates (e.g. insects and crustaceans) considered potential carriers all tested negative. Of the six representative fish species experimentally infected with TiLV, only Nile tilapia showed the typical clinical signs of the disease, with 70% mortality within 12\u00a0days. By contrast, four carp species and one catfish species challenged with TiLV showed no signs of TiLV infection. Challenged tilapia were confirmed as TiLV\u2010positive by RT\u2010qPCR, while challenged carp and walking catfish all tested negative. Overall, our field and laboratory findings indicate that species used in polycultures are not susceptible to TiLV. Although current evidence suggests that TiLV is likely host\u2010specific to tilapia, targeted surveillance for TiLV in other fish species in polyculture systems should continue, in order to prepare for a possible future scenario where TiLV mutates and\/or adapts to new host(s).<\/jats:p>","DOI":"10.1111\/jfd.13537","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T04:35:27Z","timestamp":1632803727000},"page":"77-87","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Tilapia Lake Virus was not detected in non\u2010tilapine species within tilapia polyculture systems of Bangladesh"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8107-7880","authenticated-orcid":false,"given":"Partho Pratim","family":"Debnath","sequence":"first","affiliation":[{"name":"The International Graduate Course of Veterinary Science and Technology (VST) Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"},{"name":"Department of Veterinary Microbiology Center of Excellent in Fish Infectious Diseases (CE FID) Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5318-6265","authenticated-orcid":false,"given":"Nguyen","family":"Dinh\u2010Hung","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology Center of Excellent in Fish Infectious Diseases (CE FID) Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwimon","family":"Taengphu","sequence":"additional","affiliation":[{"name":"Fish Health Platform Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Bangkok Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vuong Viet","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Research Institute for Aquaculture No Bac Ninh Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4169-2456","authenticated-orcid":false,"given":"Jerome","family":"Delamare\u2010Deboutteville","sequence":"additional","affiliation":[{"name":"WorldFish Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5599-4343","authenticated-orcid":false,"given":"Saengchan","family":"Senapin","sequence":"additional","affiliation":[{"name":"Fish Health Platform Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Bangkok Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2574-284X","authenticated-orcid":false,"given":"Chadag","family":"Vishnumurthy Mohan","sequence":"additional","affiliation":[{"name":"WorldFish Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7190-757X","authenticated-orcid":false,"given":"Ha Thanh","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Food, Agriculture and Bioresources Aquaculture and Aquatic Resources Management Program Asian Institute of Technology (AIT) School of Environment Klong Luang Pathumthani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5711-9294","authenticated-orcid":false,"given":"Channarong","family":"Rodkhum","sequence":"additional","affiliation":[{"name":"The International Graduate Course of Veterinary Science and Technology (VST) Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"},{"name":"Department of Veterinary Microbiology Center of Excellent in Fish Infectious Diseases (CE FID) Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12843"},{"key":"e_1_2_9_3_1","volume-title":"Virus Taxonomy: 2018b Release","author":"Bacharach 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