{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:12:30Z","timestamp":1787015550869,"version":"build-2736575974"},"reference-count":88,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T00:00:00Z","timestamp":1636156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin City","doi-asserted-by":"publisher","award":["17JCQNJC15000"],"award-info":[{"award-number":["17JCQNJC15000"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The half\u2010smooth tongue sole,\n                    <jats:italic>Cynoglossus semilaevis<\/jats:italic>\n                    , is an important cultured flatfish species.\n                    <jats:italic>Vibrio harveyi<\/jats:italic>\n                    is a common pathogen to this fish, which may result in great economic loss to\n                    <jats:italic>C.\u00a0semilaevis<\/jats:italic>\n                    culture industry. piRNAs, a non\u2010coding RNAs with 26\u201332\u00a0nt, have been regarded as promising biomarkers for cancer diagnosis and fish diseases. Here, we extracted the RNA from mucus of\n                    <jats:italic>C.\u00a0semilaevis<\/jats:italic>\n                    and constructed the differential expression profiles of piRNAs between the sick fish (MS) and healthy fish (MC). We identified 45,696 differentially expressed piRNAs including 22,735 up\u2010regulated piRNAs and 22,961 down\u2010regulated piRNAs in MS group compared with MC group. The GO enrichment and KEGG pathway enrichment analyses of the differential piRNAs were carried out. The result showed immunity\u2010related target genes mainly involved in immune system process, response to stimulus, cell killing, immune system, infectious diseases and cell growth and death. The 10 most differentially expressed piRNAs were chosen to perform the qRT\u2010PCR, while only seven piRNAs were consistent with the sequence result. Compared with MC group, the expression levels of piR\u2010mmu\u201072173&gt;piR\u2010rno\u201062831&gt;piR\u2010xtr\u2010704880, piR\u2010dme\u201015546979, piR\u2010mmu\u201049941660, piR\u2010mmu\u201029283297 and piR\u2010mmu\u20101758399 were significantly lower, and piR\u2010gga\u201010574 and piR\u2010gga\u2010134812 were significantly higher in MS group. These piRNAs may be potential biomarkers during the\n                    <jats:italic>V.\u00a0harveyi<\/jats:italic>\n                    infection of\n                    <jats:italic>C.\u00a0semilaevis<\/jats:italic>\n                    . This study could provide a new method to identify the infection status of\n                    <jats:italic>C.\u00a0semilaevis<\/jats:italic>\n                    and understand better about the innate and adaptive immune system in\n                    <jats:italic>C.\u00a0semilaevis<\/jats:italic>\n                    during bacterial infection.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13546","type":"journal-article","created":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T10:28:42Z","timestamp":1636194522000},"page":"165-175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mucus piRNAs profiles of\n                    <i>Vibrio harveyi<\/i>\n                    \u2010infected\n                    <i>Cynoglossus semilaevis<\/i>\n                    : A hint for fish disease monitoring"],"prefix":"10.1111","volume":"45","author":[{"given":"Na","family":"Zhao","sequence":"first","affiliation":[{"name":"Southern Marine science and engineering Guangdong laboratory\u2010Zhanjiang Guangdong Ocean University Zhanjiang China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University) Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxia","family":"Deng","sequence":"additional","affiliation":[{"name":"Southern Marine science and engineering Guangdong laboratory\u2010Zhanjiang Guangdong Ocean University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Southern Marine science and engineering Guangdong laboratory\u2010Zhanjiang Guangdong Ocean University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9157-8988","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Southern Marine science and engineering Guangdong laboratory\u2010Zhanjiang Guangdong Ocean University Zhanjiang China"},{"name":"Tianjin Fisheries Research Institute Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1011-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna5010008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.03.008"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35048692"},{"key":"e_1_2_10_6_1","first-page":"2267","volume-title":"Isolation and identification of Vibrio rotiferianus from diseased half\u2010smooth tongue sole (Cynoglossus semilaevis G\u00fcnther)","author":"Chen Z.","year":"2011"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3724\/SP.J.1231.2012.27667"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.10.004"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2011.06.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.12.008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10547"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.641052"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.02.005"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12796"},{"issue":"7","key":"e_1_2_10_15_1","first-page":"1145","article-title":"Isolation, identification and pathogenicity of pathogenic Pseudomonas fluorescens from Cynoglossus semilaevis Gunther","volume":"36","author":"Gao G. S.","year":"2016","journal-title":"Chinese Journal of Veterinary Science"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg006"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj112"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm952"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02010940"},{"key":"e_1_2_10_20_1","first-page":"178","article-title":"Research progress of Piwi\u2010interacting RNAs in Fish","volume":"24","author":"He X.","year":"2020","journal-title":"Journal of Economic Animal"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.204"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2017.09.002"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku881"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.11239"},{"key":"e_1_2_10_25_1","first-page":"19","volume-title":"piRNA dynamics in divergent zebrafish strains reveal long\u2010lasting maternal influence on zygotic piRNA profiles","author":"Kaaij L. J. T.","year":"2013"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.02.008"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-2697-z"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz130"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1107s32"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.237"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5461.2185"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2016.2671"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.02.016"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12557"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12557"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010019\u20101052\u20109"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_10_39_1","first-page":"1770","article-title":"Analyzing of heritability and breeding values for resistance Against Vibrio harveyi in Chinese tongue sole (Cynoglossus semilaevis)","volume":"26","author":"Lu S.","year":"2018","journal-title":"Journal of Agricultural Biotechnology"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.265157.120"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8316"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2010.01184.x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt310"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2019.09.003"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201069355\u20107"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2021.100864"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249439"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052373"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-757"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10672"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.11.064"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7546-4_6"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12040598"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evaa094"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.96.003"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178534"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.11.014"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.18489"},{"key":"e_1_2_10_59_1","first-page":"793","article-title":"The map\u2010based sequence of the rice genome","volume":"436","author":"Shu O.","year":"2011","journal-title":"Nature"},{"key":"e_1_2_10_60_1","first-page":"127","article-title":"Recent updates on the role of extracellular vesicles in the pathogenesis of allergic asthma","volume":"2","author":"Srinivasan A.","year":"2021","journal-title":"Extracellular Vesicles and Circulating Nucleic Acids"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20103305\u20107\u2010177"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.09.020"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11802\u2010008\u20100343\u20103"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.914222"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201010\u2010310"},{"key":"e_1_2_10_66_1","first-page":"301","article-title":"Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)","volume":"37","author":"Wang C. L.","year":"2016","journal-title":"Zoological Research"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2017.04.007"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1043"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-470"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2013.04.021"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13577\u2010020\u201000463\u20102"},{"key":"e_1_2_10_72_1","first-page":"86","article-title":"Isolation and identification of pathogen responsible for the vibriosis in cultured Cynoglossus semilaevisis","volume":"53","author":"XU X. l.","year":"2017","journal-title":"Chinese Journal of Veterinary Medicine"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597\u2010019\u20100231\u20102"},{"issue":"2014","key":"e_1_2_10_74_1","first-page":"12","article-title":"Isolation and identification of pathogen of ascites disease from Cynoglossus semilaevis G\u00fcnther and its antibiotic sensitivity analysis","volume":"21","author":"Xuan H. U.","year":"2014","journal-title":"Journal of Tianjin Agricultural University"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.135"},{"key":"e_1_2_10_76_1","first-page":"763","article-title":"Isolation of Photobacterium damselae subsp. piscicida from diseased tongue sole (Cynoglossus semilaevis Gunther) in China","volume":"47","author":"Yan W.","year":"2007","journal-title":"Acta Microbiologica Sinica"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.06.008"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002849900221"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.244830.118"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.6709"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010020\u20106660\u20107"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.4"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42995-020-00037-z"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.11.018"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.01.038"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.02.001"},{"key":"e_1_2_10_87_1","volume-title":"Identification and pathogenicity of Aeromonas strains associated with skin ulceration disease of Cynoglossus semilaevis G\u00fcnther","author":"Zhou H.","year":"2017"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2011.11.044"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.11.019"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13546","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13546","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13546","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T01:36:45Z","timestamp":1693186605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13546"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,6]]},"references-count":88,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1111\/jfd.13546"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13546","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,6]]},"assertion":[{"value":"2021-08-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}