{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:05:39Z","timestamp":1760123139971,"version":"3.40.5"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Renibacterium salmoninarum<\/jats:italic>, a slow\u2010growing facultative intracellular pathogen, is the causative agent of bacterial kidney disease, a chronic, progressive and granulomatous infection that threatens farmed and wild salmonids worldwide. Pathogenic <jats:italic>R.\u00a0salmoninarum<\/jats:italic> colonizes tissues and invades the host through cell surface\u2010associated and secreted proteins. While correlations between iron acquisition genes and virulence have been demonstrated <jats:italic>in vitro<\/jats:italic>, these mechanisms have not undergone proteomic characterization. The present study applied a proteomic approach to elucidate the differences between the virulent Chilean <jats:italic>R.\u00a0salmoninarum<\/jats:italic> H\u20102 strain and the type strain ATCC 33209<jats:sup>T<\/jats:sup>. Analyses were conducted under normal (control) and iron\u2010limited conditions (DIP) emulating the host environment. Interestingly, strain H\u20102 apparently responded better to the iron\u2010limited condition\u2014for example, only this strain presented a significantly enriched iron ion homeostasis pathway. Furthermore, key virulence factors related to an iron\u2010limited environment were more abundant in strain H\u20102. Importantly, the lack of iron favoured the expression of the 57\u2010kDa protein in strain H\u20102, the principal virulence factor for <jats:italic>R.\u00a0salmoninarum<\/jats:italic>. Our findings can be employed in the design and development of treatments targeted to iron uptake mechanisms (e.g. siderophore synthesis or haem uptake), which represents a promising therapeutic approach for treating this persistent fastidious bacterium.<\/jats:p>","DOI":"10.1111\/jfd.13554","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T08:52:23Z","timestamp":1637225543000},"page":"289-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteomic analysis reveals <i>Renibacterium salmoninarum<\/i> grown under iron\u2010limited conditions induces iron uptake mechanisms and overproduction of the 57\u2010kDa protein"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-4475","authenticated-orcid":false,"given":"Ruben","family":"Avenda\u00f1o\u2010Herrera","sequence":"first","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research (INCAR) Vi\u00f1a del Mar Chile"},{"name":"Centro de Investigaci\u00f3n Marina Quintay (CIMARQ) Universidad Andr\u00e9s Bello Quintay Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Saldivia","sequence":"additional","affiliation":[{"name":"Division of Biotechnology MELISA Institute San Pedro de la Paz, Concepci\u00f3n Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7457-4072","authenticated-orcid":false,"given":"Jorn","family":"Bethke","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research (INCAR) Vi\u00f1a del Mar Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian","family":"Vargas","sequence":"additional","affiliation":[{"name":"Division of Biotechnology MELISA Institute San Pedro de la Paz, Concepci\u00f3n Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2277-0132","authenticated-orcid":false,"given":"Mauricio","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Division of Biotechnology MELISA Institute San Pedro de la Paz, Concepci\u00f3n Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00055-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000201"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13051"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12456"},{"issue":"4","key":"e_1_2_8_6_1","first-page":"169","article-title":"Biochemical, serological, and genetic characterisation of Renibacterium salmoninarum isolates recovered from salmonids in Chile","volume":"37","author":"Bethke J.","year":"2017","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy138"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp101"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2020.118829"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-1-133"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao034223"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-5-949"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.19.5241"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13362"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.11.057"},{"key":"e_1_2_8_16_1","first-page":"511","article-title":"An improved growth medium for the kidney bacterium and some notes on using the medium","volume":"87","author":"Evelyn T. 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