{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T18:10:47Z","timestamp":1784830247948,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gilthead seabream represents a species of importance in Mediterranean aquaculture. The larval stage is particularly sensitive and frequently impacted in suboptimal environmental or sanitary conditions. In the present study, investigations were carried out in a seabream hatchery following an unusual mortality reaching 70% among 50\u2010day post\u2010hatching. Anorexia, loss of appetite and abnormal swimming behaviour were observed in absence of parasites or pathogenic bacteria. Proliferation of rod\u2010shaped bacteria in the gut lumen was associated with focal degeneration in the intestinal mucosa. Cytopathic effects on an EK\u20101 cell line after 21\u00a0days of culture at 14\u00b0C and 20\u00b0C in contact with homogenized affected larvae revealed the presence of a viral agent. Molecular characterization by high\u2010throughput sequencing showed a typical picornavirus genome organization with a polyprotein precursor of 2276 amino acids sharing 46.3% identity with that of the Eel Picornavirus\u20101. A specific real\u2010time PCR confirmed the presence of the viral genome in affected larval homogenate and corresponding cell culture supernatant. We propose the name <jats:italic>Potamipivirus daurada<\/jats:italic> for this novel species within the genus Potamipivirus. The etiological role of this virus remains uncertain at this time, and future studies will be necessary to investigate its prevalence in natural and aquaculture\u2010reared populations as well as its ability to cause diseases in gilthead seabream.<\/jats:p>","DOI":"10.1111\/jfd.13596","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T18:04:35Z","timestamp":1645034675000},"page":"707-716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of a novel picornavirus isolated from moribund gilthead seabream (<i>Sparus aurata<\/i>) larvae"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3537-7767","authenticated-orcid":false,"given":"L\u00e9na\u00efg","family":"Louboutin","sequence":"first","affiliation":[{"name":"ANSES Agence Nationale de S\u00e9curit\u00e9 Sanitaire de l\u2019Alimentation, de l\u2019Environnement et du 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