{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T20:34:15Z","timestamp":1781728455609,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42106133"],"award-info":[{"award-number":["42106133"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptococcus agalactiae<\/jats:italic>, also known as Group B Streptococcus (GBS), can infect humans, terrestrial animals and fish. The emergence of bacterial resistance of<jats:italic>S.\u00a0agalactiae<\/jats:italic>to antibiotics leads to an urgent need of exploration of new antimicrobial agents. In the study, the antibacterial activity of natural component plumbagin (PLB) against<jats:italic>S.\u00a0agalactiae<\/jats:italic>was investigated in vitro and in vivo. The results showed that the minimal inhibitory concentration (MIC) of PLB against<jats:italic>S.\u00a0agalactiae<\/jats:italic>was 8\u00a0mg\/L. The growth curve assay revealed that PLB could inhibit the growth of<jats:italic>S.\u00a0agalactiae<\/jats:italic>. In addition, the time\u2010killing curve showed that<jats:italic>S.\u00a0agalactiae<\/jats:italic>was killed almost completely by 2\u2010fold MIC of PLB within 12\u00a0h. Transmission electron microscopy results showed obvious severe morphological destruction and abnormal cells of<jats:italic>S.\u00a0agalactiae<\/jats:italic>after treated with PLB. The pathogenicity of<jats:italic>S.\u00a0agalactiae<\/jats:italic>to zebrafish was significantly decreased after preincubation with PLB for 2\u00a0h in vitro, further indicating the bactericidal activity of PLB. Interestingly, PLB could kill<jats:italic>S.\u00a0agalactiae<\/jats:italic>without inducing resistance development. Furthermore, pretreatment and post\u2010treatment assays suggested that PLB also exhibited the antibacterial activity against<jats:italic>S.\u00a0agalactiae<\/jats:italic>infection in vivo by effectively reducing the bacterial load and improving the survival rate of<jats:italic>S.\u00a0agalactiae<\/jats:italic>\u2010infected zebrafish. In summary, PLB had potent antibacterial activity against<jats:italic>S.\u00a0agalactiae<\/jats:italic>in vitro and in vivo, and it could be an excellent antimicrobial candidate to prevent and control<jats:italic>S.\u00a0agalactiae<\/jats:italic>infection.<\/jats:p>","DOI":"10.1111\/jfd.13606","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T07:29:28Z","timestamp":1647934168000},"page":"815-823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Natural component plumbagin as a potential antibacterial agent against<i>Streptococcus agalactiae<\/i>infection"],"prefix":"10.1111","volume":"45","author":[{"given":"Ai\u2010Guo","family":"Huang","sequence":"first","affiliation":[{"name":"Guangxi Laboratory on the Study of Coral Reefs in the South China Sea Guangxi University Nanning China"},{"name":"School of Marine Sciences Guangxi University Nanning China"},{"name":"College of Life 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