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HBP was predicted to be a bacterial secretory protein, located at mitochondrial membrane in eukaryotic cells and have a similar protein structure with the heme\u2010binding protein of <jats:italic>Mycobacterium tuberculosis<\/jats:italic>, Rv0203. In this study, HBP was found to be a secretory protein and co\u2010localized with mitochondria in FHM cells. Quantitative analysis of mitochondrial membrane potential value, caspase\u20103 activity, and transcription level of apoptosis\u2010related genes suggested that overexpression of HBP protein can induce cell apoptosis. In conclusion, HBP was a secretory protein which may target to mitochondria and involve in cell apoptosis in host cells. 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