{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T10:04:12Z","timestamp":1780913052575,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T00:00:00Z","timestamp":1653868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Tilapia parvovirus<\/jats:italic>\n                    (TiPV) is an emerging virus reportedly associated with disease and mortality in farmed tilapia. Although previous descriptions of histopathological changes are available, the lesions reported in these are not pathognomonic. Here, we report Cowdry type A inclusion bodies (CAIB) in the pancreas as a diagnostic histopathological feature found in adult Nile tilapia naturally infected with TiPV. This type of inclusion body has been well\u2010known as a histopathological landmark for the diagnosis of other parvoviral infections in shrimp and terrestrial species. Interestingly, this lesion could be exclusively observed in pancreatic acinar cells, both in the hepatopancreas and pancreatic tissue along the intestine. In situ hybridization (ISH) using a TiPV\u2010specific probe revealed the intranuclear presence of TiPV DNA in multiple tissues, including the liver, pancreas, kidney, spleen, gills and the membrane of oocytes in the ovary. These findings suggest that although TiPV can replicate in several tissue types, CAIB manifest exclusively in pancreatic tissues. In addition to TiPV, most diseased fish were co\u2010infected with\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    , and presented with multifocal granulomas secondary to this bacterial infection. Partial genome amplification of TiPV was successful and revealed high nucleotide identity (&gt;99%) to previously reported isolates. In summary, this study highlights the usefulness of pancreatic tissue as a prime target for histopathological diagnosis of TiPV in diseased Nile tilapia. This pattern may be critical when determining the presence of TiPV infection in new geographic areas, where ancillary testing may not be available. TiPV pathogenesis in this landmark organ warrants further investigation.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13663","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T02:05:49Z","timestamp":1653962749000},"page":"1323-1331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Usefulness of the pancreas as a prime target for histopathological diagnosis of\n                    <i>Tilapia parvovirus<\/i>\n                    (\n                    <scp>TiPV<\/scp>\n                    ) infection in Nile tilapia,\n                    <i>Oreochromis niloticus<\/i>"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7190-757X","authenticated-orcid":false,"given":"Ha Thanh","family":"Dong","sequence":"first","affiliation":[{"name":"AARM\/FAB, School of Environment, Resources and Development Asian Institute of Technology  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4173-1371","authenticated-orcid":false,"given":"Pattiya","family":"Sangpo","sequence":"additional","affiliation":[{"name":"Fish Heath Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1201-4330","authenticated-orcid":false,"given":"Le Thanh","family":"Dien","sequence":"additional","affiliation":[{"name":"Faculty of Applied Technology, School of Engineering and Technology Van Lang University  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1953-1103","authenticated-orcid":false,"given":"Thao Thu","family":"Mai","sequence":"additional","affiliation":[{"name":"Division of Aquacultural Biotechnology, Biotechnology Center of Ho Chi Minh City  Ho Chi Minh Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0812-4764","authenticated-orcid":false,"given":"Nguyen Vu","family":"Linh","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences, Faculty of Agriculture Chiang Mai University  Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9582-1228","authenticated-orcid":false,"given":"Jorge","family":"del\u2010Pozo","sequence":"additional","affiliation":[{"name":"Easter Bush Pathology, Royal (Dick) School of Veterinary Studies  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1623-0831","authenticated-orcid":false,"given":"Krishna R.","family":"Salin","sequence":"additional","affiliation":[{"name":"AARM\/FAB, School of Environment, Resources and Development Asian Institute of Technology  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5599-4343","authenticated-orcid":false,"given":"Saengchan","family":"Senapin","sequence":"additional","affiliation":[{"name":"Fish Heath Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science Mahidol University  Bangkok Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13367"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.734541"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.09.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02815"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00827\u201014"},{"key":"e_1_2_9_7_1","first-page":"244","volume-title":"The State of World Fisheries and Aquaculture 2020. 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