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The TA\u201002 cells are grown for 300\u2009days in an L\u201015 medium supplemented with 10% fetal bovine serum (FBS). This cell line showed excellent proliferative capacity and expressed various neuroglial cell markers, including SOX2, SOX10, Hes1, Notch1, Occludin, E\u2010cadherin, and GFAP. In addition, TA\u201002 cells were susceptible to <jats:italic>Tilapia Lake Virus<\/jats:italic> (TiLV) as demonstrated by the presence of a severe cytopathic effect (CPE), virus particle in a transmission electron microscope (TEM), and PCR positive signal. Bacterial cytotoxicity studies showed that <jats:italic>Streptococcus agalactiae<\/jats:italic> was toxic to TA\u201002 cells. When co\u2010culture with trans\u2010well, TA\u201002 exhibited prominent barrier properties, manifested by tight intercellular junctions and increased trans\u2010endothelial electrical resistance (TEER). In addition, the barrier is effective against <jats:italic>Escherichia coli<\/jats:italic> (non\u2010meningitis pathogenic bacteria). In contrast, <jats:italic>S. agalactiae<\/jats:italic> (meningitis pathogenic bacteria) can pass through the membrane comprising the cells in the trans\u2010well insert. The newly established TA\u201002 cell line provided a valuable tool for virus pathogenesis and a <jats:italic>vitro<\/jats:italic> model of the fish blood\u2013brain barrier.<\/jats:p>","DOI":"10.1111\/jfd.13674","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T10:21:06Z","timestamp":1656325266000},"page":"1451-1462","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Establishment of an astrocyte\u2010like cell line from the brain of tilapia (<i>Oreochromis niloticus<\/i>) for virus pathogenesis and a vitro model of the blood\u2013brain barrier"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9934-7634","authenticated-orcid":false,"given":"Yuan","family":"Li","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University  Zhanjiang P. R. China"},{"name":"Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University  Zhanjiang P. R. China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9501-7336","authenticated-orcid":false,"given":"Jia","family":"Cai","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University  Zhanjiang P. R. China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongneng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University  Zhanjiang P. R. 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