{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:58:54Z","timestamp":1778885934075,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20210101050JC"],"award-info":[{"award-number":["20210101050JC"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aeromonas veronii<\/jats:italic> is a significant pathogen that is capable of infecting humans, animals, and aquatic animals. The type III secretion system (T3SS) is intimately associated with bacterial pathogenicity. The <jats:italic>ascO<\/jats:italic> gene is an important core component of T3SS in <jats:italic>A. veronii<\/jats:italic>, but its function is still unclear. The <jats:italic>ascO<\/jats:italic> gene of <jats:italic>A. veronii<\/jats:italic> TH0426 was deleted by using the pRE112 suicide plasmid to study its function. The study results showed that the ability of \u2206<jats:italic>ascO<\/jats:italic> to adhere and invade EPC cells was significantly reduced by 1.28 times. The toxicity of the mutant strain \u0394<jats:italic>ascO<\/jats:italic> to EPC cells was consistently significantly lower than wild\u2010type strain TH0426 at 1, 2, and 4\u2009h. The LD50 values of \u2206<jats:italic>ascO<\/jats:italic> against zebrafish and <jats:italic>Carassius auratus<\/jats:italic> (<jats:italic>C. auratus<\/jats:italic>) were 53 and 15 times that of the wild\u2010type strain. In addition, the bacterial load of the mutant strain \u0394<jats:italic>ascO<\/jats:italic> in blood, heart, liver, and spleen was lower than wild\u2010type strain TH0426. The Hoechst staining showed that the apoptotic degree of EPC cells induced by the mutant strain \u0394<jats:italic>ascO<\/jats:italic> was lower than that of the wild\u2010type strain TH0426. Furthermore, real\u2010time quantitative PCR (RT\u2010qPCR) analysis revealed lower expression levels of pro\u2010apoptotic genes (including <jats:italic>cytC<\/jats:italic>, <jats:italic>cas3<\/jats:italic>, <jats:italic>cas9<\/jats:italic>, TNF\u2010\u03b1, and IL\u20101\u03b2) in <jats:italic>C. auratus<\/jats:italic> tissues infected with the mutant strain \u0394<jats:italic>ascO<\/jats:italic> compared to the wild\u2010type strain TH0426. The results of in vivo and in vitro experiments have shown that <jats:italic>ascO<\/jats:italic> gene mutation can reduce the adhesion and toxicity of <jats:italic>A. veronii<\/jats:italic> to EPC and reduce the level of apoptosis induced by <jats:italic>A. veronii<\/jats:italic>. As a result, these insights will help further elucidate the function of the <jats:italic>ascO<\/jats:italic> gene and thus contribute to understanding the pathogenesis of <jats:italic>A. veronii<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13676","type":"journal-article","created":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T18:00:57Z","timestamp":1656093657000},"page":"1477-1489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Essential role of <i>ascO<\/i> for virulence of <i>Aeromonas veronii<\/i> and inducing apoptosis"],"prefix":"10.1111","volume":"45","author":[{"given":"Tong","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine\/College of Animal Science and Technology Jilin Agricultural University  Changchun China"},{"name":"Marine College Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1709-2282","authenticated-orcid":false,"given":"Bintong","family":"Yang","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongjin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine\/College of Animal Science and Technology Jilin Agricultural University  Changchun China"},{"name":"Marine College Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aidong","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine\/College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Cong","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuwen","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine\/College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanhuan","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine\/College of Animal Science and Technology Jilin Agricultural University  Changchun China"},{"name":"Marine College Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines10050826"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.186.8.2402\u20102412.2004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.188.10.3525\u20103534.2006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113418"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.01.035"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2017.11.016"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075028"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1938449"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/Cmr.00022\u201013"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079459608419155"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.182.7.1834\u20101843.2000"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400326101"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106976.12061"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12617"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20101119(97)00619\u20107"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.176.15.4501\u20104510.1994"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0769\u20102617(83)80060\u20104"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01468"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0147\u20109571(98)00134\u20109"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.071035"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13075"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11020316"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1898777"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400391101"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3865802"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.01.018"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00177"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4595\u20104607.2003"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695\u2010016\u20100268\u20105"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287\u2010147\u20102\u2010449"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2307\/2335002"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890510037329"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06497"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.180.15.3882\u20103890.1998"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003324"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102958.2003.03772.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12020306"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.14390"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.060030097"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.01551\u201014"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.00103\u201012"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41067"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0167\u20107012(00)00122\u20106"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/Jb.00761\u201008"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17040496"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.15.3876"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.11.027"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00239"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01387"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2019.10494"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.6.1561\u20101569.1994"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.10.009"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a011623"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13172"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1513\u20101519.2003"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.040626\u20100"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.08.017"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13676","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13676","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T13:05:53Z","timestamp":1692795953000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,24]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/jfd.13676"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13676","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,24]]},"assertion":[{"value":"2022-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}