{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:11:49Z","timestamp":1787015509519,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T00:00:00Z","timestamp":1657929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32173011"],"award-info":[{"award-number":["32173011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Major capsid protein (MCP) can be used as a subunit vaccine against largemouth bass virus (LMBV). However, subunit vaccines usually have low immunogenicity. Here, to identify the major immunogenicity determinant region of the\n                    <jats:italic>MCP<\/jats:italic>\n                    gene, we truncated the\n                    <jats:italic>MCP<\/jats:italic>\n                    of the LMBV gene into four parts (\n                    <jats:italic>MCP<\/jats:italic>\n                    \u2010\n                    <jats:italic>1, MCP<\/jats:italic>\n                    \u2010\n                    <jats:italic>2, MCP<\/jats:italic>\n                    \u2010\n                    <jats:italic>3<\/jats:italic>\n                    and\n                    <jats:italic>MCP<\/jats:italic>\n                    \u2010\n                    <jats:italic>4<\/jats:italic>\n                    ). Enzyme\u2010linked immunosorbent assay (ELISA) was used to identify the antigenicity of these four truncated MCP proteins. Then, the highly antigenic truncated protein was modified with mannose and connected with functionalized single\u2010walled carbon nanotubes (SWCNTs) as carriers. Largemouth basses were immunized by bath immersion, challenged with LMBV on the 28th day after immunization and evaluated for related immune indicators. The results indicated that the MCP\u20102 protein could induce a higher antibody titre than the other truncated MCP proteins. We found that the levels of immune\u2010related genes (\n                    <jats:italic>TNF\u2010\u03b1, CD40<\/jats:italic>\n                    ,\n                    <jats:italic>IgM<\/jats:italic>\n                    ,\n                    <jats:italic>IFN\u03b3<\/jats:italic>\n                    and\n                    <jats:italic>IL\u201010<\/jats:italic>\n                    ) in the spleen and kidney were significantly increased in the MCP\u20102 and MCP\u20102\u2010Man groups. ELISA results showed that the antibody content in the serum increased significantly in the MCP\u20102 group 7\u2009days post\u2010vaccination and increased with days in all the vaccinated groups, with the highest observed on the 21st day. Notably, the MCP\u20102\u2010Man vaccine (10\u00a0mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) showed durability of immunoprotection efficacy that could protect largemouth basses from LMBV challenge, and the immune protection rate reached 78.94%. These results suggest that\n                    <jats:italic>MCP\u20102<\/jats:italic>\n                    might be the major immunogenicity determinant region of LMBV and that the mannose\u2010modified MCP\u20102 vaccine can induce stronger adaptive immune responses.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13686","type":"journal-article","created":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T13:39:20Z","timestamp":1657978760000},"page":"1635-1643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Antigenic epitope screening and functional modification of mannose enhance the efficacy of largemouth bass virus subunit vaccines"],"prefix":"10.1111","volume":"45","author":[{"given":"Yi\u2010Jun","family":"Jia","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Yao","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Yi","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5702-524X","authenticated-orcid":false,"given":"Bin","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2010.12.032"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao054029"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.02.055"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.01.015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.05.021"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.5.6.803"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.10.018"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.03.025"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2015.05.047"},{"issue":"1","key":"e_1_2_10_12_1","first-page":"68","article-title":"Prokaryotic expression and immunogenicity of dominant epitope region of goose parvovirus structural protein VP3","volume":"10","author":"Li S. G.","year":"2018","journal-title":"Animal Husbandry and Feed Science."},{"issue":"8","key":"e_1_2_10_13_1","first-page":"139","article-title":"Prokaryotic expression of MCP gene from largemouth bass ulcerative syndrome virus and the immune effect analysis of recombinant protein","volume":"32","author":"Ma D. M.","year":"2016","journal-title":"Biotechnology Bulletin"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2019.1689080"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1998.0136"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2013.190"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1577888"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1696028"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104318"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.05.006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c01401"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2008.11.023"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.641447"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.10.006"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2016.01.017"},{"issue":"3","key":"e_1_2_10_26_1","first-page":"8","article-title":"Identification of major antigenic domain of E protein of Japanese encephalitis virus","volume":"17","author":"Yan L. P.","year":"2009","journal-title":"Chinese Journal of Animal Infectious Diseases"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b09041"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aan5692"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.06.062"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.08.003"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.05.029"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108953"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.06.001"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.736197"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.09.014"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.09.063"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines8010087"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2017.07.024"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13686","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13686","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13686","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T07:39:48Z","timestamp":1692689988000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,16]]},"references-count":37,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/jfd.13686"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13686","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,16]]},"assertion":[{"value":"2022-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}