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Medicinal plants have been known for thousands of years and benefit of medicinal plants against pathogens in aquaculture have emerged. Nowadays, the most commonly used method for detecting virus infection and assessing antiviral drugs efficacy is reverse transcription\u2010quantitative real\u2010time PCR. However, the application is limited on account of high reagent costs, complex time\u2010consuming operations and long detection time. Aptamers have been widely applied in application of pathogens or diseases diagnosis and treatments because of high specificity, strong affinity, good stability, easy synthesized and low costs. This study aimed to establish an aptamer (GBN34)\u2010based high\u2010throughput screening (GBN34\u2010AHTS) model for efficient selection and evaluation of natural ingredients against NNV infection. GBN34\u2010AHTS is an expeditious rapid method for selecting natural ingredients against NNV, which is characterized with high\u2010speed, dram, sensitive and accurate. AHTS strategy could reduce work intensity and experimental costs and shorten the whole screening cycle of effective ingredients. AHTS should be suitable for rapid selection of effective ingredients against other viruses, which is important for improving the prevention and controlling of aquatic diseases.<\/jats:p>","DOI":"10.1111\/jfd.13693","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T19:44:56Z","timestamp":1659469496000},"page":"1711-1719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Study on aptamer based high throughput approach identifies natural ingredients against <scp>RGNNV<\/scp>"],"prefix":"10.1111","volume":"45","author":[{"given":"Hongling","family":"Wei","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology (GERCFT), Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological 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