{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T06:13:55Z","timestamp":1775542435687,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006462","name":"Anhui Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006462","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fish nocardiosis mainly caused by <jats:italic>Nocardia seriolae<\/jats:italic> (<jats:italic>N. seriolae)<\/jats:italic> is a serious threat to aquaculture. Bacterial adhesion to host cells mediated by adhesin is an initial step of pathogenesis. But it is not clear whether glyceraldehyde\u20103\u2010phosphate dehydrogenase (GapA) is an adhesin of <jats:italic>N. seriolae<\/jats:italic>. Here, recombinant GapA protein (rGapA) was prokaryotic expressed, and its role in the bacterial adhesion to <jats:italic>Ctenopharyngodon idella<\/jats:italic> kidney cells was investigated by indirect immunofluorescence, protein\u2010binding assay and adhesion inhibition assay. The results showed that an obvious green fluorescence was observed on the surface of the cells co\u2010incubated with rGapA protein; the cytomembrane proteins of the cells pretreated with rGapA could react with anti\u2010rGapA antibody; and the antibody significantly inhibited the adhesion ability of the bacteria. Subsequently, B\u2010cell linear epitopes of GapA protein were identified by using a immunoinformatics approach combined with peptide ELISA and Western blot for the first time. It was found that four predicted epitopes (Ep<jats:sub>58\u201069<\/jats:sub>, Ep<jats:sub>139\u2010150<\/jats:sub>, Ep<jats:sub>186\u2010197<\/jats:sub>, Ep<jats:sub>318\u2010329<\/jats:sub>) could all react with anti\u2010rGapA antibody and obviously inhibit the immunoreactivity between rGapA and anti\u2010rGapA antibody, and they were confirmed as indeed B\u2010cell linear epitopes of the protein. Furthermore, flow cytometry analysis found the percentage of positive cells co\u2010incubated with FITC\u2010labelled epitope peptides (Ep<jats:sub>139\u2010150<\/jats:sub>, Ep<jats:sub>186\u2010197<\/jats:sub>, Ep<jats:sub>318\u2010329<\/jats:sub>) was significantly higher than those in the FITC\u2010labelled Ep<jats:sub>58\u201069<\/jats:sub>, unrelated control peptide and cell control. Collectively, GapA is an adhesin of <jats:italic>N. seriolae<\/jats:italic>, and epitope peptides (Ep<jats:sub>139\u2010150<\/jats:sub>, Ep<jats:sub>186\u2010197<\/jats:sub>, Ep<jats:sub>318\u2010329<\/jats:sub>) possess cell\u2010binding activity, which are potential candidates for developing a multiple epitopes\u2010based adhesin vaccine against fish nocardiosis.<\/jats:p>","DOI":"10.1111\/jfd.13709","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T16:15:32Z","timestamp":1662048932000},"page":"1845-1855","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["First identification of <i>Nocardia seriolae<\/i><scp>GapA<\/scp> adhesion function and its three B\u2010cell epitopes with cell\u2010binding activity"],"prefix":"10.1111","volume":"45","author":[{"given":"Jiajing","family":"Guo","sequence":"first","affiliation":[{"name":"Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology Anhui Agricultural University  Hefei P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhen","family":"Yue","sequence":"additional","affiliation":[{"name":"Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology Anhui Agricultural University  Hefei P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaojiao","family":"Chang","sequence":"additional","affiliation":[{"name":"Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology Anhui Agricultural University  Hefei P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology Anhui Agricultural University  Hefei P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinnian","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology Anhui Agricultural University  Hefei P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuelan","family":"Liu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology Anhui Agricultural University  Hefei P. R. 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