{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:04:13Z","timestamp":1791331453026,"version":"4.3.1"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T00:00:00Z","timestamp":1662854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aeromonas salmonicida<\/jats:italic> is a Gram\u2010negative bacterium that can infect a wide host range of fish populations, including salmonids and non\u2010salmonids as well as freshwater and marine life. Some strains of <jats:italic>A. salmonicida<\/jats:italic> cause the disease furunculosis, which can cause lethargy, intestinal inflammation, ulcers, haemorrhaging and death. The infection is spread through fish\u2010to\u2010fish contact, and the presence of infection can have devastating effects on cultivated fish populations. The purpose of this study was to explore the ability of non\u2010A\u2010layer and A\u2010layer <jats:italic>A. salmonicida<\/jats:italic> strains to incorporate polyunsaturated fatty acids (PUFAs) into their lipid profile and test the phenotypic effects thereof. Lipids were extracted from PUFA\u2010exposed cultures and analysed for lipid modification by thin\u2010layer chromatography and ultraperformance liquid chromatography\u2010mass spectrometry, showing <jats:italic>A. salmonicida<\/jats:italic>, regardless of A\u2010layer, capable of incorporating all seven of the PUFAs studied. Phenotypic effects were determined through the use of assays that tested for biofilm formation, membrane permeability and cyclic peptide susceptibility. Temperature\u2010dependent effects on biofilm formation were observed, and PUFA exposure showed significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) increases in membrane permeability as tested by the uptake of the hydrophobic compounds crystal violet and ethidium bromide. Additionally, some PUFAs elicited modest protection and vulnerability against the membrane\u2010targeting cyclic peptides polymyxin B (PMB) and colistin. The diverse, strain\u2010specific responses to exogenous PUFAs may allude to evolved adaptive strategies that enhance survival, persistence and virulence of non\u2010pathogenic and pathogenic members of bacteria that oscillate between environmental and fish host niches.<\/jats:p>","DOI":"10.1111\/jfd.13715","type":"journal-article","created":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T11:50:00Z","timestamp":1662897000000},"page":"31-45","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Exogenous polyunsaturated fatty acids (<scp>PUFAs<\/scp>) influence permeability, antimicrobial peptide resistance, biofilm formation and membrane phospholipid structure in an A\u2010layer and <scp>non\u2010A<\/scp>\u2010layer strain of <i>Aeromonas salmonicida<\/i>"],"prefix":"10.1111","volume":"46","author":[{"given":"Rachel N.","family":"Hofer","sequence":"first","affiliation":[{"name":"Department of Chemistry and Physics The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3442-746X","authenticated-orcid":false,"given":"Allen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biology, Geology, and Environmental Science The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7427-6657","authenticated-orcid":false,"given":"Benjamin C.","family":"House","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher N.","family":"Purvis","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0213-5975","authenticated-orcid":false,"given":"Bradley J.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5741-439X","authenticated-orcid":false,"given":"Steven J. K.","family":"Symes","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8419-9122","authenticated-orcid":false,"given":"David K.","family":"Giles","sequence":"additional","affiliation":[{"name":"Department of Biology, Geology, and Environmental Science The University of Tennessee at Chattanooga  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.1986.tb01042.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010018\u20105100\u20104"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010018\u20101259\u20108"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2005.00617.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59\u2010099"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.000059"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2021.101093"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6809\u20106814.2004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12516"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.24.7968\u20107975.1993"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201091670\u2010w"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.11.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2017.09.010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0809.020063"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305\u20100491(88)90130\u20107"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000556"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2021.110325"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010020\u201001988\u20100"},{"key":"e_1_2_8_20_1","volume-title":"Fish diseases and disorders, Volume 3: Viral, bacterial and fungal infections","author":"Hiney M.","year":"1999"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.635"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044\u20100305(00)00159\u20108"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044\u20100305(01)00285\u20109"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.03.246"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/625023"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0306\u20103623(97)00305\u20104"},{"issue":"5","key":"e_1_2_8_27_1","first-page":"412","article-title":"Form and functions of the regular surface array (S\u2010layer) of Aeromonas salmonicida","volume":"47","author":"Kay W. W.","year":"1991","journal-title":"Experientia"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/09637480701777886"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010019\u20100297\u20106"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2012.737474"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531127"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2017.00073"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn123"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03006"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13063791"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0954422408102414"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01415\u201017"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.12949"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.593\u2010656.2003"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106968.1997.tb12616.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42447-1"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2015.09.016"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.3791\/2437"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01875"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42770\u2010019\u201000180\u20105"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.1985.tb03319.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.09.024"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044\u20108486(99)00083\u20106"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03645\u201013"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1237"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/are.15512"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00037"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2014\/6883.4032"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35617"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.7b00170"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.29.6.992"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/124.7.1133"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R114.636241"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/679109"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050858"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800005\u2010JLR200"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13715","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13715","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13715","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T03:23:34Z","timestamp":1692588214000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,11]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/jfd.13715"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13715","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,11]]},"assertion":[{"value":"2022-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}