{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:13:55Z","timestamp":1790046835126,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T00:00:00Z","timestamp":1665273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Largemouth bass ranavirus (LMBRaV), also known as largemouth bass virus (LMBV), is a high mortality pathogen in largemouth bass. A rapid, sensitive, specific and convenient diagnosis method is an urgent requirement for the prevention of virus transmission. In the present study, a droplet digital PCR (ddPCR) method based on the major capsid protein (\n                    <jats:italic>mcp<\/jats:italic>\n                    ) gene was established to detect and quantify the virus genome copy number. Oligonucleotide primers were designed based on the LMBRaV\n                    <jats:italic>mcp<\/jats:italic>\n                    gene sequence. The specificity and sensitivity of ddPCR assay were analysed. The other aquatic virus including Chinese giant salamander iridovirus (GSIV), Cyprinid herpesvirus II (CyHV\u20102) and infectious spleen and kidney necrosis virus could not be detected by LMBRaV ddPCR assay. The detection limit of ddPCR assay was 2\u2009\u00b1\u20090.37 copies\/\u03bcl DNA sample. And this ddPCR assay had great repeatability and reproducibility. In clinical diagnosis of 50 largemouth bass, 43 positive samples were detected by ddPCR, whereas only 34 positive samples were detected by quantitative PCR (qPCR). This LMBRaV detection assay provided a specific and sensitive method for the rapid diagnosis of LMBRaV infection in largemouth bass as well as quantification of the virus load.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13721","type":"journal-article","created":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T07:54:56Z","timestamp":1665302096000},"page":"91-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Development of a droplet digital\n                    <scp>PCR<\/scp>\n                    method for the sensitive detection and quantification of largemouth bass ranavirus"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8460-3949","authenticated-orcid":false,"given":"Nan","family":"Jiang","sequence":"first","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Shen","sequence":"additional","affiliation":[{"name":"Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province Zhejiang Institute of Freshwater Fisheries  Huzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhi","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Meng","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqun","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3370-5712","authenticated-orcid":false,"given":"Mingyang","family":"Xue","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuding","family":"Fan","sequence":"additional","affiliation":[{"name":"Division of Fish Disease, Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.12.012"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journa.pone.0092476"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2010.12.032"},{"key":"e_1_2_9_5_1","first-page":"28","article-title":"Development of droplet digital PCR method for detection of classical swine fever virus","volume":"29","author":"Gao X.","year":"2021","journal-title":"Chinese Journal of Animal Infectious Diseases"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/08997659.2014.922517"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1577\/H07\u2010009.1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao050233"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548\u20108446(2003)28[10:ROLBV]2.0.CO;2"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.08.019"},{"key":"e_1_2_9_11_1","first-page":"59","volume-title":"Ranaviruses: Lethal pathogens of ectothermic vertebrates","author":"Jancovich J. 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