{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:40:19Z","timestamp":1791841219381,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A2065"],"award-info":[{"award-number":["U20A2065"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Astragalus<\/jats:italic> polysaccharides (APS) and <jats:italic>Ganoderma lucidum<\/jats:italic> polysaccharides (GLP) have been shown to possess strong immunoregulatory properties in aquatic animals. In this study, the fragment containing <jats:italic>Vibrio harveyi flgJ<\/jats:italic> gene was ligated into pcDNA3.1(+) vector and pcDNA3.1(+)\u2010flgJ was constructed as DNA vaccine. APS and GLP were used as DNA vaccine adjuvants to evaluate the immunoregulatory effect by intramuscular injection to pearl gentian grouper (<jats:bold>\u2640<\/jats:bold><jats:italic>Epinephelus fuscoguttatus<\/jats:italic> \u00d7 \u2642<jats:italic>E. lanceolatus<\/jats:italic>). The results showed that pcDNA3.1(+)\u2010flgJ combined with APS or GLP could significantly up\u2010regulate the innate and adaptive immune response in fish, including serum\u2010specific antibody titres, catalase and lysozyme activities. At the same time, DNA vaccine combined with APS or GLP significantly up\u2010regulated the expression levels of <jats:italic>CD8\u03b1<\/jats:italic>, <jats:italic>IgM<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic>, <jats:italic>MHC\u2010I\u03b1<\/jats:italic>, <jats:italic>MyD88<\/jats:italic> and <jats:italic>TLR3<\/jats:italic> genes in thymus, head kidney, spleen and liver of pearl gentian grouper in comparison with those of the pFlgJ group. After 42\u2009days post\u2010vaccination, <jats:italic>V. harveyi<\/jats:italic> was used to challenge pearl gentian grouper by intraperitoneal injection. The relative percentage of survival (RPS) of pFlgJ, pFlgJ +APS, pFlgJ +GLP and pFlgJ+APS+GLP groups were 69%, 81%, 77% and 88%, respectively. These results suggested APS and GLP were potential adjuvants for DNA vaccine against <jats:italic>V. harveyi<\/jats:italic> infection in pearl gentian grouper.<\/jats:p>","DOI":"10.1111\/jfd.13728","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T06:58:27Z","timestamp":1668063507000},"page":"147-156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Immune\u2010enhancing effects of <i>Astragalus<\/i> polysaccharides and <i>Ganoderma lucidum<\/i> polysaccharides on <i>Vibrio harveyi flgJ<\/i> DNA vaccine in grouper"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7395-7573","authenticated-orcid":false,"given":"Guixiang","family":"Lin","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture &amp; Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Fisheries College of Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Da","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture &amp; Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Fisheries College of Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4183-6977","authenticated-orcid":false,"given":"Xiaoju","family":"Wan","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture &amp; Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Fisheries College of Guangdong Ocean University  Zhanjiang 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