{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:07:20Z","timestamp":1781114840198,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011798","name":"Ministry of Agriculture and Rural Affairs of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011798","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The infectious spleen and kidney necrosis virus (ISKNV) is a highly lethal virus, which has brought significant losses to aquaculture. Therefore, a new vaccine against ISKNV with high efficiency, safety and convenience must be developed. While baculoviruses are more commonly used as protein expression systems for vaccine antigen production, this paper used baculovirus technology to develop a live\u2010vector vaccine, BacMCP, which contains the coding sequence of the major capsid protein (MCP) (GenBank accession no. AF371960) of ISKNV and is driven by a CMV promoter. Real\u2010time PCR and immunofluorescence showed that the MCP gene was successfully delivered to and expressed in fish cells and tissues inoculated with BacMCP. Immune\u2010related gene (IgM, TGF\u2010\u03b2, IL\u20101, IL\u20108, TNF\u2010\u03b1) expression was induced in BacMCP\u2010treated groups of largemouth bass compared with control groups. Specific antibodies could be detected in the serum of BacMCP injection\u2010vaccinated largemouth bass by ELISA. After injection or immersion vaccination with BacMCP for 21\u2009days, largemouth bass were infected with ISKNV. The immune effect of the injected immunization on fish in different sizes was evaluated. The vaccine efficacy of injection\u2010vaccinated bass was 100% in small bass and 85.7% in large bass. The vaccine efficacy of immersion\u2010vaccinated small bass was 77.3%. This study suggested that BacMCP can be used as a vector\u2010based vaccine candidate to prevent the diseases caused by ISKNV infection.<\/jats:p>","DOI":"10.1111\/jfd.13731","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T22:36:24Z","timestamp":1669329384000},"page":"165-176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A recombinant baculovirus vector vaccine (<scp>BacMCP<\/scp>) against the infectious spleen and kidney necrosis virus (<scp>ISKNV<\/scp>)"],"prefix":"10.1111","volume":"46","author":[{"given":"Min","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"},{"name":"Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeen","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchao","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Tong","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"},{"name":"Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"},{"name":"Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujun","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation Beibu Gulf University  Qinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangli","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"},{"name":"Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renyu","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"},{"name":"Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6545-727X","authenticated-orcid":false,"given":"Chengliang","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Biology and Basic Medical Sciences Soochow University  Suzhou China"},{"name":"Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00110-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.08.036"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252312000175"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00494-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.07.012"},{"key":"e_1_2_10_7_1","first-page":"388","article-title":"Immune effect of recombinant major capsid protein of infectious spleen and kidney necrosis virus from Siniperca chuatsi","volume":"16","author":"Fu X. 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