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In this study, monoclonal antibodies (mAbs) against MRV were produced and characterized, and 7 mAbs were obtained through Western blotting screening and all 7 mAbs specifically recognized MRV\/LMBV but not several piscine iridoviruses as ISKNV, GIV and TFV. By LC MS\/MS analysis, the recognized viral proteins by seven mAbs were identified as MRV\u2010pORF47L, MRV\u2010pORF55R, MRV\u2010pORF57L, MRV\u2010pORF77L and MRV\u2010pORF78L, respectively, and all five viral proteins are late expression structural proteins by Western blotting. Based on mAb 1C4, immuno\u2010histochemistry and immuno\u2010histo\u2010fluorescence were performed to re\u2010assess the tissue tropism of MRV. The result showed that abundant reactive signals were observed in infected spleen, kidney as well as intestine and pyloric caecum. Real\u2010time quantitative PCR also demonstrated that spleen as well as pyloric caecum and intestines are the major target tissue upon MRV infection. In infected intestines and pyloric caecum, numerous enlarged, multinucleated cells with intracytoplasmic inclusions were identified as the target cells of MRV, suggesting that MRV serves as a digestive tract pathogen to mandarinfish, which may explain why acute infection of MRV can cause the typical clinicopathology featured by severe ascites.<\/jats:p>","DOI":"10.1111\/jfd.13733","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T18:57:48Z","timestamp":1669661868000},"page":"189-199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Production and characterization of monoclonal antibodies against mandarinfish ranavirus and first identification of pyloric caecum as the major target tissue"],"prefix":"10.1111","volume":"46","author":[{"given":"Wenfeng","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Bio\u2010control, School of Life Sciences Sun Yat\u2010sen University  Guangzhou P.R. 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