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A lateral flow immuno\u2010chromatographic assay (LFIA) using two specific anti\u2010CyHV\u20103 monoclonal antibodies has been developed and validated for on\u2010site detection of CyHV\u20103. MAb 3C9 was used to bio\u2010conjugate CyHV\u20103 antigen with colloidal gold, and MAb 2A8 was used to capture antigen bound colloidal gold on the test line. The control line was lined with goat anti\u2010mouse IgG to capture unbound colloidal gold to validate performance. The test results can be viewed within 10\u2009min after putting the strip into CyHV\u20103 virus infection fluid. The lowest limit of detection for the LFIA test was found to be 1.5\u2009\u00d7\u200910<jats:sup>4<\/jats:sup> copies\/\u03bcL and it showed no cross\u2010reactivity with other fish viral pathogens. The specificity of the strip was 100% when spleen and kidney tissues of CyHV\u20103\u2010infected and healthy koi were validated at the field level. The LFIA strip will be an effective device for the early detection of CyHV\u20103 in the future.<\/jats:p>","DOI":"10.1111\/jfd.13825","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T08:04:44Z","timestamp":1688630684000},"page":"1065-1071","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of a lateral flow immuno\u2010chromatic strip assay for the detection of cyprinid herpesvirus 3 (<scp>CyHV<\/scp>\u20103)"],"prefix":"10.1111","volume":"46","author":[{"given":"Yihua","family":"Zheng","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8116-7627","authenticated-orcid":false,"given":"Yi","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lupin","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxun","family":"Li","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5665-7157","authenticated-orcid":false,"given":"Liqun","family":"Lu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"},{"name":"Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean University  Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yousheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"},{"name":"Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean University  Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00146\u201007"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8AN00307F"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u20105\u201013"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.09.025"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.01.023"},{"key":"e_1_2_8_7_1","unstructured":"Chen J. 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