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Therefore, in this study, a nitrite removal strain XA19 was isolated and screened from a shrimp biofloc culture pond. This strain was identified as a clade of <jats:italic>Vibrio proteolyticus<\/jats:italic> because the homology between XA19 and <jats:italic>V. proteolyticus WDVP<\/jats:italic> was as high as 99.86% by using 16S rDNA gene sequence analysis and NCBI database comparison. Scanning electron microscopy images showed that <jats:italic>V. proteolyticus<\/jats:italic> is short\u2010rod\u2010shaped with a curved body and no budding spores, pods and flagella. Antimicrobial susceptibility test proved that <jats:italic>V. proteolyticus<\/jats:italic> was resistant to ampicillin, oxacillin, penicillin, vancomycin and clindamycin. In the median lethal concentration 50 (LC<jats:sub>50<\/jats:sub>) test, at 7\u2010day post\u2010infection (dpi), LC<jats:sub>50<\/jats:sub> of <jats:italic>V. proteolyticus<\/jats:italic> for <jats:italic>Fenneropenaeus merguiensis<\/jats:italic> was 1.69\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>\u2009CFU\/mL. Transcriptome sequencing analysis was carried out on hepatopancreas of <jats:italic>F. merguiensis<\/jats:italic> at 24 and 48\u2009hpi. A total of 176 differentially expressed genes (DEGs) were screened at 24\u2009hpi, including 104 up\u2010regulated DEGs and 72 down\u2010regulated DEGs, and a total of 52 DEGs were screened at 48\u2009hpi, including 32 up\u2010regulated DEGs and 20 down\u2010regulated DEGs. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs, many immune\u2010related signalling pathways were significantly enriched, including Hippo signalling pathway, phagosome, Toll and Imd signalling pathways and Wnt signalling pathway. In addition, some pathways related to Warburg effect were also enriched, including Glycolysis\/Gluconeogenesis, Biosynthesis of amino acids, amino sugar and nucleotide sugar metabolism and so on. In this study, the toxicity and drug sensitivity of <jats:italic>V. proteolyticus<\/jats:italic> were systematically studied, and the immune response of hepatopancreas of <jats:italic>F. merguiensis<\/jats:italic> to <jats:italic>V. proteolyticus<\/jats:italic> infection was preliminarily revealed from the molecular level. The results may provide a reference for the prevention and control of <jats:italic>V. proteolyticus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13840","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T15:03:37Z","timestamp":1692630217000},"page":"1207-1224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptome analysis of <i>Fenneropenaeus merguiensis<\/i> in response to <i>Vibrio proteolyticus<\/i> infection"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9377-0308","authenticated-orcid":false,"given":"Yunqi","family":"Zhong","sequence":"first","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"He","sequence":"additional","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Long","sequence":"additional","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danqing","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianye","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbo","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Fisheries Guangdong Ocean University  Zhanjiang China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals  Zhanjiang China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang)  Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2021.107545"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.02.029"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2021.111747"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1939.tb06990.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15716"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05385-11"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27732"},{"issue":"4","key":"e_1_2_11_9_1","first-page":"43","article-title":"Molecular mechanisms of fluoroquinolone and expanded\u2010spectrum cephalosporin resistance in Vibrio parahaemolyticus: Abridged secondary publication","volume":"26","author":"Chen S.","year":"2020","journal-title":"Hong Kong Medical Journal"},{"key":"e_1_2_11_10_1","article-title":"Metagenomic analysis of shrimp biofloc culture pond and screening and evaluation of highly efficient denitrify bacteria","author":"Chen X. 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