{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:38:06Z","timestamp":1790735886947,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:00:00Z","timestamp":1695513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Edwardsiella piscicida<\/jats:italic>, an infectious bacterium, causes great economic losses to the aquaculture industry. Immersion bath which is the closest way to how the fish infect bacterial pathogens in the natural environment is an effective route of artificial infection. In this study, the dynamic process of <jats:italic>E.\u2009piscicida<\/jats:italic> infection, in the spotted sea bass (<jats:italic>Lateolabrax maculatus<\/jats:italic>) was evaluated via the immersion bath. The results showed that soaking the spotted sea bass with 3\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009CFU\u2009mL<jats:sup>\u22121<\/jats:sup> <jats:italic>E.\u2009piscicida<\/jats:italic> for 30\u2009min could artificially induce edwardsiellosis. The higher culture temperature (28.5\u2009\u00b1\u20090.5\u00b0C) or the longer bath time (30\u2009min) would lead to higher mortality of fish. <jats:italic>E.piscicida<\/jats:italic> first invaded the gill, then entered the blood circulation to infect the spleen and kidney, where it is colonized, and gradually multiplied in the liver and brain. Meanwhile, the fluorescence in situ hybridization showed that the localization of <jats:italic>E.\u2009piscicida<\/jats:italic> in the gill and foregut after the immersion challenge proceeded from the exterior to the interior. The invasion of pathogens triggers the immune response of fish and causes tissue damage to the host. The quantitative real\u2010time PCR results displayed an increase in the relative expression level of immune genes (<jats:italic>NK\u2010lysin<\/jats:italic>, <jats:italic>LZM<\/jats:italic>, <jats:italic>IgM<\/jats:italic> and <jats:italic>IgD<\/jats:italic>). Otherwise, the most notable histopathological changes of the infected spotted sea bass were multifocal necrosis. Findings in this study broaden our understanding of the infection conditions of <jats:italic>E.\u2009piscicida<\/jats:italic> and its pathogenicity to the spotted sea bass.<\/jats:p>","DOI":"10.1111\/jfd.13863","type":"journal-article","created":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T05:09:51Z","timestamp":1695618591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The pathway of <i>Edwardsiella piscicida<\/i> infecting <i>Lateolabrax maculatus<\/i> via the immersion bath"],"prefix":"10.1111","volume":"47","author":[{"given":"Jianmei","family":"Hu","sequence":"first","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology Zhongkai University of Agriculture and Engineering  Guangzhou China"},{"name":"Key Laboratory of South China Sea Fishery Resources Development and Utilization Ministry of Agriculture and Rural Affairs  Guangzhou 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