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In the present study, <jats:italic>Aeromonas caviae<\/jats:italic> and <jats:italic>A. veronii<\/jats:italic> were isolated from four diseased European seabass (<jats:italic>Dicentrarchus labrax<\/jats:italic>) farms experiencing a high mortality rate. Diseased fish showed haemorrhages on the external body surface with exophthalmia, cataracts, scale desquamation, skin ulcers and fin erosions. The most common post\u2010mortem findings were congested internal organs, particularly the liver and posterior kidney. Twenty\u2010eight <jats:italic>A. Veronii<\/jats:italic> and 11 <jats:italic>A. caviae<\/jats:italic> isolates were identified biochemically by the Vitek 2 system and then confirmed by PCR and phylogenetic analysis. Hemolysin (<jats:italic>hlyA<\/jats:italic>) and aerolysin (<jats:italic>aer<\/jats:italic>) were the most abundant virulence genes in the recovered isolates, followed by cytotoxic enterotoxin (<jats:italic>act<\/jats:italic>) and heat\u2010stable enterotoxin (<jats:italic>ast<\/jats:italic>). <jats:italic>A. caviae<\/jats:italic> was more virulent than <jats:italic>A. veronii<\/jats:italic> for <jats:italic>D. labrax<\/jats:italic> fingerlings as LD<jats:sub>50<\/jats:sub> ranging between (&gt;1\u2009\u00d7\u200910<jats:sup>8<\/jats:sup>\u20136.2\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>) for <jats:italic>A. veronii<\/jats:italic> and (2.9\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\u20138.3\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>) for <jats:italic>A. caviae<\/jats:italic>. The sensitivity test indicated the effectiveness of norfloxacin, doxycycline and oxytetracycline against the tested isolates. Serum cortisol significantly increased in the infected groups, while catalase and glutathione peroxidase activities significantly decreased at 2\u2009days post\u2010infection (DPI) and then increased at 6 DPI. The presence of virulence genes was associated with bacterial pathogenicity expressed in fish mortality rate. Virulence genes also drastically affect cortisol levels more than catalase and glutathione peroxidase levels.<\/jats:p>","DOI":"10.1111\/jfd.13878","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T06:02:15Z","timestamp":1698300135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The role of <i>Aeromonas<\/i> genotyping in virulence for <i>Dicentrarchus labrax<\/i>"],"prefix":"10.1111","volume":"47","author":[{"given":"Ibrahim Mohamed","family":"Aboyadak","sequence":"first","affiliation":[{"name":"National Institute of Oceanography and Fisheries, NIOF  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdy Khalil","family":"Soliman","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Damanhour University  Damanhour Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadeer Mohamed","family":"Nageeb","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Damanhour University  Damanhour Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-0014","authenticated-orcid":false,"given":"Nadia Gabr","family":"Ali","sequence":"additional","affiliation":[{"name":"National Institute of Oceanography and Fisheries, NIOF  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10499\u2010019\u201000429\u20108"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012373944-5.00022-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.19080\/JPCR.2016.01.555573"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.15406\/jamb.2015.02.00045"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4172\/2155\u20109546.1000496"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737107"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738373"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2021.717360"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-32674-0"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10111192"},{"key":"e_1_2_10_12_1","volume-title":"Java for bioinformatics and biomedical applications","author":"Bal H.","year":"2007"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.06.011"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14986"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009987"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2010.02.002"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13028\u2010020\u20100504\u2010y"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-7345.2004.tb00105.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517\u2010838220120001000046"},{"key":"e_1_2_10_20_1","first-page":"276","volume-title":"Mediterranean yearbook","author":"Carvalho N.","year":"2021"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026042125243"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.06.049"},{"key":"e_1_2_10_23_1","volume-title":"Document M45\u2010A. 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