{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:53:36Z","timestamp":1783612416007,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Nocardia seriolae<\/jats:italic> is the primary aetiological agent of nocardiosis in fish, which causes mass mortality in freshwater and marine fish. \u03b2\u2010ketoacyl\u2010ACP synthase (KAS) is one of the essential enzymes in the synthesis of mycolic acids (MASs) in <jats:italic>Mycobacterium<\/jats:italic> spp. and has been chosen as the target for therapeutic intervention in mycobacterial diseases. In the present study, a <jats:italic>kas<\/jats:italic>B homologue gene (<jats:italic>kasB<\/jats:italic>) was identified in the genome of <jats:italic>N.\u2009seriolae<\/jats:italic>, and the gene\u2010deficient mutant (\u0394<jats:italic>kasB<\/jats:italic>) was generated based on a clinical isolate, XSYC\u2010Ns. Compared to the wild\u2010type (WT) strain, the \u0394<jats:italic>kasB<\/jats:italic> showed a measurably growth defect in\u00a0vitro but retained the acid\u2010fastness in acid\u2010fast staining. Observation of the cell ultrastructure showed some alterations in the cell wall of the \u0394<jats:italic>kasB<\/jats:italic> strain. Compared to its original strain, the cell wall lipid layer seemed sparser, and a wider electron\u2010transparent zone was observed in the cell wall of \u0394<jats:italic>kasB<\/jats:italic> strain. Moreover, the \u0394<jats:italic>kasB<\/jats:italic> strain showed impaired ability of cell invasion as well as intracellular survival in the cell line originating from the head\u2010kidney of the large yellow croaker (LYC\u2010hK), compared to its original strain. In addition, the deficiency of \u0394<jats:italic>kasB<\/jats:italic> significantly attenuated the virulence of <jats:italic>N. seriolae<\/jats:italic> in largemouth bass. The present study suggested that the \u0394<jats:italic>kasB<\/jats:italic> gene might be involved in the synthesis of extracellular cell\u2010wall lipids in <jats:italic>N. seriolae<\/jats:italic> and play a crucial role in its pathogenicity.<\/jats:p>","DOI":"10.1111\/jfd.13896","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T17:52:58Z","timestamp":1701885178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Disruption of a <i>kasB<\/i> homolog gene (<i>kasB<\/i>) causes attenuation of cell invasion and virulence of <i>Nocardia seriolae<\/i>"],"prefix":"10.1111","volume":"47","author":[{"given":"Su\u2010Ming","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng\u2010Ling","family":"Shu","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Qian","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7255-7321","authenticated-orcid":false,"given":"Jia\u2010Song","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0539-4876","authenticated-orcid":false,"given":"Fei","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory of the Ministry of Education for Applied Marine Biotechnology School of Marine Science, Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0168\u20101656(03)00163\u20109"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608654104"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.22.7596\u20107606.2005"},{"issue":"2","key":"e_1_2_10_5_1","first-page":"2656","article-title":"Establishment of a specific PCR method for detection of Nocardia seriolae","volume":"40","author":"Chen G.","year":"2021","journal-title":"Genomics and Applied Biology"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102761.2000.00217.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01419\u201017"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106968.12597"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.11.012"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102958.2003.03667.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13154"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009\u20103084(93)90015\u2010u"},{"key":"e_1_2_10_13_1","first-page":"297","volume-title":"Diseases in Asian aquaculture VI","author":"Labrie L.","year":"2008"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03517"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12782"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2013.11.011"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13737"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00446\u201012"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004115"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03023"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2007.06.019"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2005.00637.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12962"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2011.11.016"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnx143"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2012.02.006"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738866"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13896","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T09:39:02Z","timestamp":1707385142000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13896"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,6]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/jfd.13896"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13896","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,6]]},"assertion":[{"value":"2023-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13896"}}