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This bacterium was identified as <jats:italic>E.\u2009roggenkampii<\/jats:italic> based on the phenotypic characteristics and sequence analysis of the16S rDNA and <jats:italic>gyrB<\/jats:italic> genes. Average nucleotide identity and phylogenetic analysis based on the whole genome sequence further confirmed the bacterial taxonomy of EOBSR_19. Artificial experimental infection indicated that EOBSR_19 was pathogenic to fish. Antimicrobial susceptibility test showed it was multi\u2010drug resistant. The EOBSR_19 was found to be resistant to 18 antibiotics belonging to quinolones, macrolides, sulfonamides, aminoglycosides, and \u03b2\u2010lactams classes. The whole genome sequencing analysis showed that EOBSR_19 carried 730 virulence genes that were annotated for different functional modules, such as adhesion and invasion, secretion system, siderophore transport system and bacterial toxin. Among them, the virulence genes related to adhesion and invasion were the most abundant. In addition, drug resistance genes involved in multiple mechanisms of antimicrobial resistance were identified in its genomics, including multidrug resistance efflux pumps, antibiotic inactivating enzymes, and antibiotic binding site mutations. Its genomic analysis via whole\u2010genome sequencing provided insights into the pathogenicity and antimicrobial resistance.<\/jats:p>","DOI":"10.1111\/jfd.13898","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T14:27:54Z","timestamp":1701181674000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Phenotypic and genomic insights into the pathogenicity and antimicrobial resistance of an <i>Enterobacter roggenkampii<\/i> strain isolated from diseased silver arowana (<i>Osteoglossum bicirrhosum<\/i>)"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0946-5594","authenticated-orcid":false,"given":"Yuerui","family":"Li","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Aqua\u2010ecology and Aquaculture, Fisheries College Tianjin Agricultural University  Tianjin 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