{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T17:34:03Z","timestamp":1782495243855,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T00:00:00Z","timestamp":1702944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJTR20U5"],"award-info":[{"award-number":["JPMJTR20U5"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The live attenuated vaccine <jats:styled-content style=\"fixed-case\">P7\u2010P8<\/jats:styled-content> strain against herpesviral haematopoietic necrosis, which is caused by cyprinid herpesvirus 2 (<jats:styled-content style=\"fixed-case\">CyHV<\/jats:styled-content>\u20102), exhibits high protective efficacy in goldfish at 25\u00b0C, the predominant temperature for this disease; however, the effect of water temperature during the vaccination period on efficacy has not been determined. In this study, an <jats:italic>in vitro<\/jats:italic> experiment revealed that the vaccine strain grew between 15 and 30\u00b0C in the goldfish cell line <jats:styled-content style=\"fixed-case\">RyuF<\/jats:styled-content>\u20102. Subsequent <jats:italic>in vivo<\/jats:italic> efficacy tests were conducted with vaccination temperatures ranging from 15 to 30\u00b0C. During the vaccination period, organs were sampled to determine the vaccine growth dynamics. Blood plasma was collected to assess <jats:styled-content style=\"fixed-case\">anti\u2010CyHV<\/jats:styled-content>\u20102 antibody titres. The protective efficacy of the vaccine at 15, 20, 25, and 30\u00b0C after subsequent virulent <jats:styled-content style=\"fixed-case\">CyHV<\/jats:styled-content>\u20102 challenge resulted in a relative percentage survival of 73.3%, 77.8%, 100%, and 77.8%, respectively, which indicated that the vaccine is effective over this temperature range. The vaccine virus load in the spleen was lowest at 15\u00b0C (10<jats:sup>3.7<\/jats:sup> <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> copies\/mg) and highest at 25\u00b0C (10<jats:sup>6.5<\/jats:sup> <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> copies\/mg). This indicates that the vaccine virus load over 10<jats:sup>4<\/jats:sup> <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> copies\/mg may elicit sufficient acquired immunity. No significant differences in antibody titre were observed between groups, which suggests that cell\u2010mediated immunity can be fundamentally involved in protection.<\/jats:p>","DOI":"10.1111\/jfd.13906","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T06:43:36Z","timestamp":1703054616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of temperature on the protective efficacy of a live attenuated vaccine against herpesviral haematopoietic necrosis in goldfish"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3165-0340","authenticated-orcid":false,"given":"Hiroaki","family":"Saito","sequence":"first","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shungo","family":"Minami","sequence":"additional","affiliation":[{"name":"Saitama Fisheries Research Institute  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manami","family":"Yuguchi","sequence":"additional","affiliation":[{"name":"Yatomi Station, Freshwater Resources Research Center Aichi Fisheries Research Institute  Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiko","family":"Shitara","sequence":"additional","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5102-6831","authenticated-orcid":false,"given":"Hidehiro","family":"Kondo","sequence":"additional","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goshi","family":"Kato","sequence":"additional","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7148-1329","authenticated-orcid":false,"given":"Motohiko","family":"Sano","sequence":"additional","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology6040039"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12734"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12400"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12550"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8030445"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.34.209"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02535"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12048"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19323\u20100"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao069137"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879801000415"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15081746"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02759"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2007.00847.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.1995.tb00296.x"},{"issue":"3","key":"e_1_2_11_17_1","first-page":"85","article-title":"Pathogenicity and biological characteristics of CyHV\u20102","volume":"35","author":"Liang L. 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