{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:51:23Z","timestamp":1791942683656,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T00:00:00Z","timestamp":1705104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aeromonas jandaei<\/jats:italic> is a gram\u2010negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft\u2010shell turtle (<jats:italic>Pelodiscus sinensis<\/jats:italic>), then named strain JDP\u2010FX. This isolate was identified as <jats:italic>A. jandaei<\/jats:italic> after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and <jats:italic>gyrB<\/jats:italic> gene sequences. Virulence genetic testing further detected temperature\u2010sensitive protease (<jats:italic>eprCAI<\/jats:italic>), type III secretion system (TTSS) (<jats:italic>ascv<\/jats:italic>), nuclease (<jats:italic>nuc<\/jats:italic>), cytotonic enterotoxin (<jats:italic>alt<\/jats:italic>) and serine proteinase (<jats:italic>ser<\/jats:italic>) in JDP\u2010FX. Compared with healthy Chinese soft\u2010shell turtle, the serum levels of total protein (TP), albumin (ALB) and globulin (GLB) were significantly decreased in the diseased Chinese soft\u2010shell turtle, while, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased. Histopathological observations showed that multiple tissues, including intestinal mucosa, liver and kidney, were severely damaged in the diseased Chinese soft\u2010shell turtle. Moreover, the diseased Chinese soft\u2010shell turtle had significant cell degeneration, necrosis, sloughing and interstitial inflammatory cell infiltration. The pathogenicity of JDP\u2010FX was tested via artificial infection. The median lethal dosage (LD<jats:sub>50<\/jats:sub>) of the strain was 1.05\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> colony forming units (CFU\/g) per weight of Chinese soft\u2010shell turtle. Drug susceptibility analysis revealed that JDP\u2010FX was susceptible to ceftazidime, minocycline, cefoperazone, ceftriaxone and piperacillin. In addition, JDP\u2010FX was resistant to doxycycline, florfenicol, sulfonamides, gentamicin, ampicillin and neomycin. Therefore, this study may provide guidance for further research into the diagnosis, prevention and treatment of JDP\u2010FX infection.<\/jats:p>","DOI":"10.1111\/jfd.13919","type":"journal-article","created":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T08:25:18Z","timestamp":1705134318000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Isolation, identification and characterization of <i>Aeromonas jandaei<\/i> from diseased Chinese soft\u2010shell turtles"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1222-5536","authenticated-orcid":false,"given":"Mengmeng","family":"Chen","sequence":"first","affiliation":[{"name":"Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences  Wuhan China"},{"name":"College of Fisheries and Life Science Shanghai Ocean University  Shanghai 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