{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:22:06Z","timestamp":1776014526701,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to investigate whether a defensin\u2010like antimicrobial peptide (C\u201013326 peptide) identified in <jats:italic>Hermetia illucens<\/jats:italic> could possess protective effect against multidrug\u2010resistant <jats:italic>Aeromonas schubertii<\/jats:italic> in hybrid snakehead (<jats:italic>Channa maculate<\/jats:italic> \u2640\u2009<jats:italic>\u00d7\u2009Channa argus<\/jats:italic> \u2642). The cDNA of C\u201013326 peptide comprised 243 nucleotides encoding 80 amino acids, with six conserved cysteine residues and the classical CS\u03b1\u03b2 structure. The recombinant expression plasmid pPIC9K\u2010C\u201013326 was constructed and transformed into GS115 <jats:italic>Pichia pastoris<\/jats:italic>, and the C\u201013326 peptide was expressed by induction with 1% methanol. The crude extract of C\u201013326 peptide was precipitated by ammonium sulfate, assayed by Braford method, detected by tricine\u2010SDS\u2010PAGE, evaluated by BandScan software and identified by liquid chromatography\u2010mass spectrometry. The C\u201013326 peptide was shown to have inhibitory activity against the growth of multidrug\u2010resistant <jats:italic>A. schubertii<\/jats:italic> DM210910 by using the minimum growth inhibitory concentration and Oxford cup method. In addition, scanning electron microscopy analysis suggested that C\u201013326 peptide inhibited the growth of <jats:italic>A. schubertii<\/jats:italic> DM210910 by damaging the bacterial cell membrane. To explore the role of peptide C\u201013326 in\u00a0vivo, hybrid snakehead was fed with peptide C\u201013326 as feed additives for 7\u2009days. The results revealed that C\u201013326 peptide could significantly down\u2010regulate the expression levels of <jats:italic>IL\u20101\u03b2<\/jats:italic>, <jats:italic>IL\u20108<\/jats:italic>, <jats:italic>IL\u201012<\/jats:italic> and <jats:italic>TNF\u2010\u03b1<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and significantly improved the survival rate of hybrid snakehead after challenging with <jats:italic>A. schubertii<\/jats:italic> DM210910. Therefore, the C\u201013326 peptide is a promising antimicrobial agent for <jats:italic>A. schubertii<\/jats:italic> treatment in aquaculture.<\/jats:p>","DOI":"10.1111\/jfd.13922","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T06:54:02Z","timestamp":1704956042000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A novel defensin\u2010like peptide C\u201013326 possesses protective effect against multidrug\u2010resistant <i>Aeromonas schubertii<\/i> in hybrid snakehead (<i>Channa maculate<\/i> \u2640\u2009\u00d7\u2009<i>Channa argus<\/i> \u2642)"],"prefix":"10.1111","volume":"47","author":[{"given":"Huanrong","family":"He","sequence":"first","affiliation":[{"name":"Innovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology Zhongkai University of Agriculture and Engineering 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