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The shrimp with an average body weight of 0.50\u2009\u00b1\u20090.09\u2009g were reared in containers with a volume of 2.5\u2009L, 21\u2009units, and a density of 20 shrimp L<jats:sup>\u22121<\/jats:sup>. The shrimp were cultured for 5\u2009days, with each treatment including biofloc system maintenance with a C\/N ratio of 10 and a control treatment without biofloc, followed by a challenge test through immersion using <jats:italic>V<\/jats:italic>. <jats:italic>parahaemolyticus<\/jats:italic> at densities of 10<jats:sup>3<\/jats:sup>, 10<jats:sup>5<\/jats:sup>, and 10<jats:sup>7<\/jats:sup>\u2009CFU\u2009mL<jats:sup>\u22121<\/jats:sup> initially. The results of the in\u00a0vitro experiment showed that biofloc suspension can inhibit and disperse biofilm formation, as well as reduce the exo\u2010enzyme activity (amylase, protease, and chitinase) of <jats:italic>V<\/jats:italic>. <jats:italic>parahaemolyticus<\/jats:italic>. Furthermore, the biofloc treatment significantly reduced the expression of the QS regulatory gene OpaR, the PirB toxin gene, and the virulence factor genes T6SS1 and T6SS2 in both in\u00a0vitro and in\u00a0vivo. The biofloc system also increased the expression of shrimp immunity\u2010related genes (LGBP, proPO, SP, and PE) and the survival rate of white shrimp challenged with <jats:italic>V<\/jats:italic>. <jats:italic>parahaemolyticus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13932","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T17:04:02Z","timestamp":1708362242000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Unveiling the positive impact of biofloc culture on <i>Vibrio parahaemolyticus<\/i> infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity"],"prefix":"10.1111","volume":"47","author":[{"given":"Widanarni","family":"Widanarni","sequence":"first","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries and Marine Sciences Bogor Agricultural University  Bogor West Java Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhamad","family":"Gustilatov","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries and Marine Sciences Bogor Agricultural University  Bogor West Java Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Ekasari","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries and Marine Sciences Bogor Agricultural University  Bogor West Java Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pande Gde Sasmita","family":"Julyantoro","sequence":"additional","affiliation":[{"name":"Department of Aquatic Resources Management, Faculty of Marine Science and Fisheries University of Udayana  Denpasar Bali Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana Elizabeth","family":"Waturangi","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology Atma Jaya Catholic University of Indonesia  Jakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-8329","authenticated-orcid":false,"given":"Sukenda","family":"Sukenda","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries and Marine Sciences Bogor Agricultural University  Bogor West Java Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10499-016-0108-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12236"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2012.08.019"},{"key":"e_1_2_10_5_1","volume-title":"Biofloc technology. 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