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In the present study, comparative proteome analysis was performed on serum samples from <jats:italic>Vibrio<\/jats:italic>\u2010resistant and <jats:italic>Vibrio<\/jats:italic>\u2010susceptible grouper. Samples were analysed using high\u2010throughput LC\u2010MS\/MS and identified 2770 unique peptides that corresponded to 344 proteins. Subsequent analysis identified 21 proteins that were significantly up\u2010regulated in the resistant group compared to the control and the susceptible groups. Those proteins are associated with immunostimulatory effects, signalling and binding cascade, metabolism, and maintaining tissue integrity and physiological condition. Besides, potential protein biomarkers related to the immune system were identified, which could be associated with the disease\u2010resistant phenotype. These data provide insights into the underlying immune mechanism of hybrid groupers upon <jats:italic>Vibrio<\/jats:italic> sp. infection.<\/jats:p>","DOI":"10.1111\/jfd.13940","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T05:40:10Z","timestamp":1711345210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in <i>Vibrio<\/i>\u2010resistant hybrid grouper, <i>Epinephelus fuscoguttatus<\/i> \u2640\u2009\u00d7\u2009<i>Epinephelus lanceolatus<\/i> \u2642"],"prefix":"10.1111","volume":"47","author":[{"given":"Nurhikmah","family":"Abu\u00a0Aziz","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science Universiti Putra Malaysia (UPM)  Serdang Selangor 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