{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:21:52Z","timestamp":1788996112104,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,24]],"date-time":"2024-03-24T00:00:00Z","timestamp":1711238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007841","name":"Council of Agriculture","doi-asserted-by":"publisher","award":["111AS\u20105.2.3\u2010BQ\u2010B1"],"award-info":[{"award-number":["111AS\u20105.2.3\u2010BQ\u2010B1"]}],"id":[{"id":"10.13039\/100007841","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018811","name":"National Pingtung University of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018811","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nocardiosis, caused by <jats:italic>Nocardia seriolae<\/jats:italic>, has been a prominent disease in Southeast Asian aquaculture in the last three decades. This granulomatous disease reported in various fish species is responsible for significant economic losses. This study investigated the pathogenicity of <jats:italic>N. seriolae<\/jats:italic> in three cultured species in Taiwan: Nile tilapia (omnivore), milkfish (herbivore) and Asian seabass (carnivore). Administration of an infective dose of 1\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009CFU\/ fish in tilapia, seabass and milkfish demonstrated mortalities of 100%, 90% and 75%, respectively. Additionally, clinical signs namely, granuloma and lesions displayed varying intensities between the groups and pathological scores. Polymerase chain reaction (PCR) amplification specific for <jats:italic>N. seriolae<\/jats:italic> was confirmed to be positive (432\u2009bp) using NS1\/NG1 primers. Post\u2010mortem lesions revealed the absence of granulomas in tilapia and milkfish and their presence in the seabass. Interestingly, the gut in tilapia showed an influx of eosinophils suggesting its role during the acute stages of infection. However, post\u2010challenge, surviving milkfish exhibited granulomatous formations, while surviving seabass progressed toward healing and tissue repair within sampled tissues. Overall, in conclusion, these results demonstrate the versatility in the immunological ability of individual Perciformes to contain this pathogen as a crucial factor that influences its degree of susceptibility.<\/jats:p>","DOI":"10.1111\/jfd.13947","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T05:51:50Z","timestamp":1711345910000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative pathogenicity of <i>Nocardia seriolae<\/i> in Nile tilapia (<i>Oreochromis niloticus<\/i>), milkfish (<i>Chanos chanos<\/i>) and Asian seabass (<i>Lates calcarifer<\/i>)"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0497-8084","authenticated-orcid":false,"given":"Sandra Celenia","family":"Nazareth","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3339-7740","authenticated-orcid":false,"given":"Li\u2010Wu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7986-1625","authenticated-orcid":false,"given":"Pei\u2010Chi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Southern Taiwan Fish Diseases Research Centre National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Research Centre for Fish Vaccine and Diseases, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2885-9370","authenticated-orcid":false,"given":"Shih\u2010Chu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"College of Veterinary Medicine, Southern Taiwan Fish Diseases Research Centre National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Research Centre for Fish Vaccine and Diseases, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"General Research Service Centre National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,24]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Banno J. &Amar E.(1984).The culture of seabass.SEAFDEC Leganes Brackishwater Station Annual Report 1\u20135."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.123.3.1235\u20101253.1975"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.2.213"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100854.2000.010306.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20210469"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111144"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.12.032"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpol.2014.03.026"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.09.038"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102761.2000.00217.x"},{"key":"e_1_2_10_12_1","volume-title":"The state of world fisheries and aquaculture 2020. 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