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To evaluate the use of high\u2010iron diets to prevent or reverse channel catfish (<jats:italic>Ictalurus punctatus<\/jats:italic>) anaemia of unknown causes, we investigated the expression of these iron\u2010regulatory genes and proteins in channel catfish fed plant\u2010based diets. Catfish fingerlings were fed five diets supplemented with 0 (basal), 125, and 250\u2009mg\/kg of either inorganic iron or organic iron for 2\u2009weeks. Ferritin, transferrin, and transferrin receptor I and II mRNA and protein expression levels in fish tissues (liver, intestine, trunk kidney, and head kidney) and plasma were determined. Transferrin (iron transporter) and TfR (I and II) genes were generally highly expressed in fish fed the basal diet compared to those fed the iron\u2010supplemented diets. In contrast, ferritin (iron storage) genes were more expressed in the trunk kidney of fish fed the iron\u2010supplemented diets than in those fed the basal diet. Our results demonstrate that supplementing channel catfish plant\u2010based diets with iron from either organic or inorganic iron sources affected the expression of the iron\u2010regulatory genes and increased body iron status in the fish.<\/jats:p>","DOI":"10.1111\/jfd.13953","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T05:35:22Z","timestamp":1713159322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>Plasma and tissue transferrin and ferritin<\/scp>, <scp>and gene expression of ferritin<\/scp>, <scp>transferrin<\/scp>, <scp>and transferrin receptors<\/scp> I <scp>and II in channel<\/scp><scp>catfish<\/scp><i>Ictalurus punctatus<\/i><scp>fed diets with different concentrations of inorganic or organic iron<\/scp>"],"prefix":"10.1111","volume":"47","author":[{"given":"Isaac","family":"Buyinza","sequence":"first","affiliation":[{"name":"University of Arkansas at Pine Bluff Department of Aquaculture and Fisheries  Pine Bluff Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace","family":"Ramena","sequence":"additional","affiliation":[{"name":"University of Arkansas at Pine Bluff Department of Aquaculture and Fisheries  Pine Bluff Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9211-1538","authenticated-orcid":false,"given":"Rebecca","family":"Lochmann","sequence":"additional","affiliation":[{"name":"University of Arkansas at Pine Bluff Department of Aquaculture and Fisheries  Pine Bluff Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Sinha","sequence":"additional","affiliation":[{"name":"University of Arkansas at Pine Bluff Department of Aquaculture and Fisheries  Pine Bluff Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Jones","sequence":"additional","affiliation":[{"name":"University of Arkansas at Pine Bluff Department of Aquaculture and Fisheries  Pine Bluff Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,14]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119946045.ch33"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(01)00063-2"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.7861\/clinmed.2020-0582"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(97)00291-3"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c120016"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-9200-6_8"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1300\/J028v03n01_08"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1546-5098(11)31004-7"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695\u2010023\u201001183\u20108"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02677"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00712"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641269609388579"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02180278"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.51.5.648"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737884"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(86)90143-2"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i35.4725"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-4079(19)33422-3"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.01.001"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1990.35.5.1002"},{"key":"e_1_2_13_22_1","first-page":"91","volume-title":"Effect of enriched diet with iron in culture of yellowtail","author":"Ikeda Y.","year":"1973"},{"key":"e_1_2_13_23_1","first-page":"259","volume-title":"Clinical biochemistry of domestic animals","author":"Kaneko J. 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