{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:19:23Z","timestamp":1785907163872,"version":"3.56.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Edwardsiella anguillarum<\/jats:italic>, a highly virulent species within the <jats:italic>Edwardsiella<\/jats:italic> genus, causes significant mortality in milkfish farms in Taiwan. This study aimed to investigate the comparison of milkfish susceptibility, a newly identified host species in Taiwanese aquaculture, with other species Nile tilapia (<jats:italic>Oreochromis niloticus<\/jats:italic>) and Asian seabass (<jats:italic>Lates calcarifer<\/jats:italic>), to <jats:italic>E. anguillarum<\/jats:italic>, elucidating its pathogenicity across both seawater and freshwater aquaculture environments. The results showed milkfish exhibited the highest mortality rate of 85% within 48\u2009h of infection, whereas Nile tilapia exhibited a mortality rate of 70% between the second\u2010 and tenth\u2010day post challenge, and seabass exhibited a mortality rate of 25% between the second\u2010 and sixth\u2010day post challenge. Gross lesions observed in milkfish included splenomegaly and haemorrhage, whereas Nile tilapia exhibited signs of ascites, exophthalmia and brain haemorrhage. Seabass displayed spleen granulomas and haemorrhage at the injection site. Histopathological analysis revealed common features across all three species, including multifocal necrosis, bacterial presence in the necrotic areas, serositis and oedema. Asian seabass also exhibited chronic lesions in the form of splenic granulomas. This study highlights the high susceptibility of milkfish and Nile tilapia to <jats:italic>E. anguillarum<\/jats:italic>, emphasizing the urgent need for further investigation into targeted vaccine development for these fish species. These results not only deepen our understanding of the differing levels of pathogenicity among the three species but also offer valuable insights for improving disease prevention and management strategies in aquaculture, including those applied within polyculture systems and for the maintenance of aquaculture water environments.<\/jats:p>","DOI":"10.1111\/jfd.13982","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T09:37:37Z","timestamp":1718876257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparative pathogenicity and histopathological analysis of <i>Edwardsiella anguillarum<\/i> intraperitoneal infection in milkfish (<i>Chanos chanos<\/i>), Nile tilapia (<i>Oreochromis niloticus<\/i>) and Asian seabass (<i>Lates calcarifer<\/i>)"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4706-9198","authenticated-orcid":false,"given":"Atiek","family":"Rahmawaty","sequence":"first","affiliation":[{"name":"International College, International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3339-7740","authenticated-orcid":false,"given":"Li\u2010Wu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7986-1625","authenticated-orcid":false,"given":"Pei\u2010Chi","family":"Wang","sequence":"additional","affiliation":[{"name":"International College, International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Department of Veterinary Medicine, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Research Centre for Fish Vaccine and Diseases, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Southern Taiwan Fish Diseases Research Centre, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2885-9370","authenticated-orcid":false,"given":"Shih\u2010Chu","family":"Chen","sequence":"additional","affiliation":[{"name":"International College, International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Department of Veterinary Medicine, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Research Centre for Fish Vaccine and Diseases, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Southern Taiwan Fish Diseases Research Centre, College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"General Research Service Center National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12080"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13058"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2018.05.006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2017.09.004"},{"issue":"1","key":"e_1_2_10_6_1","first-page":"6","article-title":"Detection of Edwardsiella tarda infection of catfish (Clarias gariepinus) in central Tapanuli regency, North Sumatra, Indonesia","volume":"13","author":"Butar\u2010Butar O. D.","year":"2020","journal-title":"IOSR Journal of Agriculture and Veterinary Science (IOSR\u2010JAVS)"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13743"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24119636"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2024.740682"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10203"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13583"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548\u20108667(2000)012<0255:HAPOEI>2.0.CO;2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638716637639"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472\u2010765X.2011.03103.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.10\u20100252"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00141"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12172"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2008.03779.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.08.005"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12576"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.40.133"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00014"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2006.00685.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2021.105292"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297\u20109716\u201043\u201067"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.03.015"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.08.028"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00053"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13688"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00970\u201017"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2014.10.008"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10169"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000322"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2011.05166.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036987"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T13:54:00Z","timestamp":1723125240000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,20]]},"references-count":34,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/jfd.13982"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13982","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,20]]},"assertion":[{"value":"2024-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13982"}}