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The aim of this proteomic study was to determine how iron limitation affects the protein expression of <jats:italic>Tenacibaculum dicentrarchi<\/jats:italic>, with a primary focus on virulence factors and proteins associated with iron uptake. The proteomic analysis was carried out using two strains of <jats:italic>T.\u2009dicentrarchi<\/jats:italic> grown under normal (control) and iron\u2010limited conditions, mimicking the host environment. Our findings revealed differences in the proteins expressed by the type strain CECT 7612<jats:sup>T<\/jats:sup> and the Chilean strain TdCh05 of <jats:italic>T.\u2009dicentrarchi<\/jats:italic>. Nonetheless, both share a common response to iron deprivation, with an increased expression of proteins associated with iron oxidation and reduction metabolism (e.g., SufA, YpmQ, SufD), siderophore transport (e.g., ExbD, TonB\u2010dependent receptor, HbpA), heme compound biosynthesis, and iron transporters under iron limitation. Proteins involved in gliding motility, such as GldL and SprE, were also upregulated in both strains. A negative differential regulation of metabolic proteins, particularly those associated with amino acid biosynthesis, was observed under iron limitation, reflecting the impact of iron availability on bacterial metabolism. Additionally, the TdCh05 strain exhibited unique proteins associated with gliding motility machinery and phage infection control compared to the type strain. These groups of proteins have been identified as virulence factors within the <jats:italic>Flavobacteriaceae<\/jats:italic> family, including the genus <jats:italic>Tenacibaculum<\/jats:italic>. These results build upon our previous report on iron acquisition mechanisms and could lay the groundwork for future studies aimed at elucidating the role of some of the described proteins in the infectious process of tenacibaculosis, as well as in the development of potential vaccines.<\/jats:p>","DOI":"10.1111\/jfd.13984","type":"journal-article","created":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T12:09:46Z","timestamp":1719662986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Proteomic characterization of <i>Tenacibaculum dicentrarchi<\/i> under iron limitation reveals an upregulation of proteins related to iron oxidation and reduction metabolism, iron uptake systems and gliding motility"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-4475","authenticated-orcid":false,"given":"Ruben","family":"Avenda\u00f1o\u2010Herrera","sequence":"first","affiliation":[{"name":"Universidad Andr\u00e9s Bello, Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida  Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research (INCAR)  Vi\u00f1a del Mar Chile"},{"name":"Centro de Investigaci\u00f3n Marina Quintay (CIMARQ) Universidad Andr\u00e9s Bello  Quintay Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9241-8006","authenticated-orcid":false,"given":"Macarena","family":"Echeverr\u00eda\u2010Bugue\u00f1o","sequence":"additional","affiliation":[{"name":"Universidad Andr\u00e9s Bello, Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida  Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research (INCAR)  Vi\u00f1a del Mar Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Division of Biotechnology Molecular Epidemiology for Life of Science Research (MELISA) Institute  San Pedro de Paz Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Saldivia","sequence":"additional","affiliation":[{"name":"Division of Biotechnology Molecular Epidemiology for Life of Science Research (MELISA) Institute  San Pedro de Paz Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-0060","authenticated-orcid":false,"given":"Rute","family":"Irgang","sequence":"additional","affiliation":[{"name":"Universidad Andr\u00e9s Bello, Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida  Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research (INCAR)  Vi\u00f1a del Mar Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.03.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/010900\u20100"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20106445(03)00055\u2010X"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13213"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12464"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12767"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13820"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11"},{"key":"e_1_2_8_10_1","unstructured":"Blighe K. 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