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Here, we tested a range of commercial FAEEs of various carbon chain lengths and unsaturation levels against two fish parasites. Guppies and barramundi infected with <jats:italic>G.\u2009turnbulli<\/jats:italic> and <jats:italic>Trichodina<\/jats:italic> sp., respectively, were used. The most effective FAEE, after excluding those toxic to fish, was ethyl laurate (12:0). For both parasites, the LD50 was 18.75\u2009\u03bcM within 250\u2009min of incubation. Ethyl eicosapentaenoate (20:5<jats:italic>n<\/jats:italic>3) was the next most effective FAEE against <jats:italic>G.\u2009turnbulli<\/jats:italic>, and dihomo\u2010\u03b3\u2010linolenic acid ethyl ester (20:3<jats:italic>n<\/jats:italic>6) and ethyl \u03b1\u2010linolenate (18:3<jats:italic>n<\/jats:italic>3) were the next most effective against <jats:italic>Trichodina<\/jats:italic> sp. In addition, FAEEs prepared from the microalga <jats:italic>Phaeodactylum tricornutum<\/jats:italic> residue, after fucoxanthin extraction, were examined against <jats:italic>Trichodina<\/jats:italic> sp. infection in barramundi for the first time. LD85 and LD100 was achieved at 2.5 and 5\u2009\u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup> of the FAEE preparation, respectively. In\u00a0vivo, immersion of infected barramundi in 1.25\u2009\u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup> of this preparation for 24\u2009h reduced infection prevalence from 100% to 53% and was non\u2010toxic to fish.<\/jats:p>","DOI":"10.1111\/jfd.13991","type":"journal-article","created":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T08:29:03Z","timestamp":1719649743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the use of fatty acid ethyl esters as a potential natural solution for the treatment of fish parasitic diseases"],"prefix":"10.1111","volume":"47","author":[{"given":"Arunjyothi","family":"Jawaji","sequence":"first","affiliation":[{"name":"The French Associates Institute for Agriculture and Biotechnology of Drylands, The J. 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