{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:12:19Z","timestamp":1770329539849,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,7]],"date-time":"2024-07-07T00:00:00Z","timestamp":1720310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY23C190001"],"award-info":[{"award-number":["LY23C190001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Displaying antigens on yeast surface as an oral vaccine has been widely explored, while its potential as an immersion vaccine has not been evaluated. Here, an immersion vaccine was prepared by displaying ORF25 of Cyprinid herpesvirus 2 (CyHV\u20102) on the surface of <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. <jats:italic>Carassius auratus gibelio<\/jats:italic> was immersion immunized by 2\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\u2009CFU\/mL yeast for 2\u2009h, and reinforce the immunity using the same method 14\u2009days after the first immunization. The results showed that ORF25 specific antibody in immunized crucian carp serum was detected at a high level, and the mRNA expression level of <jats:italic>IgM<\/jats:italic>, <jats:italic>IgT<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic>, and <jats:italic>IFN\u20101<\/jats:italic> in vaccinated head\u2010kidney and spleen tissues were higher than the control group, indicating that innate and adaptive immunity were induced. Moreover, the immersion vaccination provided effective protection for fish against CyHV\u20102, leading to a relative percent survival of 50.2%. Meanwhile, immersion vaccination restrained virus replication and histological damage in CyHV\u20102 infected crucian carp. Our data suggested that immersion immunization of <jats:italic>S.\u2009cerevisiae<\/jats:italic>\u2010displayed ORF25 could be served as a candidate vaccine to prevent CyHV\u20102 infection.<\/jats:p>","DOI":"10.1111\/jfd.13996","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T05:49:12Z","timestamp":1720417752000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immersion immunization with recombinant <i>Saccharomyces cerevisiae<\/i> displaying <scp>ORF25<\/scp> induced protective immunity against cyprinid herpesvirus 2"],"prefix":"10.1111","volume":"47","author":[{"given":"Maoxia","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6363-6928","authenticated-orcid":false,"given":"Qianjin","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0543-0109","authenticated-orcid":false,"given":"Jianfei","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9389-520X","authenticated-orcid":false,"given":"Jiong","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2019.12.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12633"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7120627"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222010932"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.08.036"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.932722"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1040336"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1577\/H05-007.1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1995.tb00296.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.12.063"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foz007"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/76494"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1505.05041"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2020.03.013"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.03.007"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2021.104079"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.12.016"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1577\/H08-045.1"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11010186"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-018-9408-x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.07.004"},{"key":"e_1_2_10_23_1","first-page":"648","article-title":"Prior exposure to ciprofloxacin disrupts intestinal homeostasis and predisposes ayu (Plecoglossus altivelis) to subsequent Pseudomonas plecoglossicida\u2010induced infection","volume":"43","author":"Wu X. Y.","year":"2022","journal-title":"Zoological Research"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.05.025"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2011-9"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2020.113877"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1913"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.03.001"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.11.012"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T19:06:14Z","timestamp":1729191974000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,7]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/jfd.13996"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13996","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,7]]},"assertion":[{"value":"2024-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13996"}}