{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:12:07Z","timestamp":1785456727091,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Spiroplasma eriocheiris<\/jats:italic> is a pathogen in the Chinese mitten crab <jats:italic>Eriocheir sinensis<\/jats:italic> and is associated with tremor disease. <jats:italic>S.\u00a0eriocheiris<\/jats:italic> infects virtually all artificially bred crustaceans, resulting in significant economic losses to the aquaculture industry in China. Recombinase polymerase amplification (RPA) is considered a promising technology for the rapid and sensitive detection of disease. For the rapid detection of <jats:italic>S. eriocheiris<\/jats:italic>, this study established basic RPA, RPA\u2010EXO and RPA\u2010LFD, which were visualised via agarose gel electrophoresis, SYBR Green I, fluorescence signals and test lines on lateral flow dipsticks. Specific primers and probes were designed based on the 16S ribosomal RNA sequence of <jats:italic>S. eriocheiris<\/jats:italic>. The optimal primer pairs for the basic RPA, RPA\u2010EXO and RPA\u2010LFD were RPA\u2010F3\/R3, RPA\u2010F3\/R3 and RPA\u2010LFD\u2010F3\/R3, respectively. The optimal temperatures and times of basic RPA, RPA\u2010EXO and RPA\u2010LFD were 37\u00b0C for 25\u2009min, 39\u00b0C for 30\u2009min and 39\u00b0C for 10\u2009min, respectively. The specificity test indicated that in addition to the positive test results for <jats:italic>S. eriocheiris<\/jats:italic>, the other five DNA templates tested negative, demonstrating strong specificity. The results of the sensitivity test revealed that the detection limits of RPA, RPA\u2010EXO and RPA\u2010LFD were 5.77\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>, 5.77\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup> and 1.52\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u2009ng\/\u03bcL, respectively, indicating high sensitivity. This study established three methods for the detection of <jats:italic>S. eriocheiris<\/jats:italic> that are characterised by a constant temperature, rapid response, high sensitivity and strong specificity.<\/jats:p>","DOI":"10.1111\/jfd.14031","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T11:44:25Z","timestamp":1732189465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of Three Recombinase Polymerase Amplification Assays for the Rapid Visual Detection of <i>Spiroplasma eriocheiris<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"Yunji","family":"Xiu","sequence":"first","affiliation":[{"name":"School of Marine Science and Engineering Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixin","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongrui","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyue","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Marine Science and Engineering Qingdao Agricultural University  Qingdao 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